Organ Transplant Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Billary and Pancreatic Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Dis Markers. 2020 Feb 13;2020:7318703. doi: 10.1155/2020/7318703. eCollection 2020.
Seizure-related 6 homolog-like 2 (), which is localized on the cell surface, has been found to be associated with tumor angiogenesis and lung cancer progression. However, the role of in hepatocellular carcinoma (HCC) is still unclear. We obtained data from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) to investigate expression and regulation in HCC. Then, HCC tissue samples were collected to verify by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical staining (IHC). Patient information was collected for survival and prognosis analysis. qRT-PCR, IHC, and bioinformatics analysis showed that the SEZ6L2 protein was highly expressed in HCC samples. Clinical data showed that high SEZ6L2 protein expression was correlated with tumor-node-metastasis (TNM) stages ( = 0.046), tumor number ( = 0.016), and tumor size ( = 0.029). Meanwhile, SEZ6L2 overexpression was closely associated with poor overall survival and disease-free survival in HCC patients. Moreover, is an independent prognostic predictor for the survival of HCC patients. This study suggests a significant correlation between and HCC, which means that may potentially serve as a useful prognostic biomarker for HCC patients.
SEZ6L2 与肿瘤血管生成和肺癌进展有关，其定位于细胞膜上。然而，在肝细胞癌（HCC）中， 的作用尚不清楚。我们从癌症基因组图谱（TCGA）和基因表达综合数据库（GEO）中获取数据，以研究 HCC 中 的表达和调控。然后，收集 HCC 组织样本，通过实时定量聚合酶链反应（qRT-PCR）和免疫组织化学染色（IHC）来验证 的表达。收集患者信息进行生存和预后分析。qRT-PCR、IHC 和生物信息学分析表明，SEZ6L2 蛋白在 HCC 样本中高表达。临床数据显示，SEZ6L2 蛋白高表达与肿瘤-淋巴结-转移（TNM）分期（ = 0.046）、肿瘤数量（ = 0.016）和肿瘤大小（ = 0.029）有关。同时，SEZ6L2 过表达与 HCC 患者的总生存期和无病生存期密切相关。此外， 是 HCC 患者生存的独立预后预测因子。本研究提示 与 HCC 之间存在显著相关性，这意味着 可能成为 HCC 患者有用的预后生物标志物。
