Wang Ziming, Lai Jiaming, Liang Lijian, Yin Xiaoyu, Wang Qian, Cheng Quanyong, Zheng Chaoxu
Department of Pancreato-Biliary Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Center for Private Medical Service & Healthcare, The First affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Transl Cancer Res. 2020 Nov;9(11):6768-6779. doi: 10.21037/tcr-20-1527.
With the feature of destructive and biliary malignancy, intrahepatic cholangiocarcinoma (ICC), presents unclear molecular mechanisms which contributes to typically poor prognosis for patients. () is a gene that encodes for a seizure-associated protein localized on the cell surface. Thus far, the function of in ICC has not been reported.
We used data from The Cancer Genome Atlas and the Gene Expression Omnibus to analyze dynamics behind and levels of expression of in ICC. Then we used qRT-PCR and Immunohistochemical staining to detect levels of expression of and thereby determined the potential clinical significance of this protein in ICC.
According to qRT-PCR and immunohistochemical analysis results, was overexpressed in ICC. Kaplan-Meier and Cox proportional hazard analyses indicated that patients afflicted by ICC with high levels of relative expression of have a poorer prognosis and that may be an independent prognostic factor which enables to the accurate prediction of overall survival (OS) and disease-free survivals' (DFS) expected rates. Subcutaneous xenograft models used to explore the role of in tumor formation in vivo. The dynamics of the gene being promote angiogenesis in cholangiocarcinoma are related to increasing expressive growth factors which include , , and the activation of the pathway.
Our findings suggest that can serve as an advanced biomarker that can be used to accurately predict a patient prognosis and be used as a target for ICC treatment.
肝内胆管癌（ICC）具有破坏性和胆管恶性肿瘤的特征，其分子机制尚不清楚，这通常导致患者预后较差。（）是一个编码位于细胞表面的癫痫相关蛋白的基因。迄今为止，尚未报道其在ICC中的功能。
我们使用来自癌症基因组图谱和基因表达综合数据库的数据来分析ICC中（）的表达动态和水平。然后我们使用qRT-PCR和免疫组织化学染色来检测（）的表达水平，从而确定该蛋白在ICC中的潜在临床意义。
根据qRT-PCR和免疫组织化学分析结果，（）在ICC中过表达。Kaplan-Meier和Cox比例风险分析表明，（）相对表达水平高的ICC患者预后较差，并且（）可能是一个独立的预后因素，能够准确预测总生存期（OS）和无病生存期（DFS）的预期率。皮下异种移植模型用于探索（）在体内肿瘤形成中的作用。（）基因在胆管癌中促进血管生成的动态与包括（）、（）、（）在内的生长因子表达增加以及（）通路的激活有关。
我们的研究结果表明，（）可作为一种先进的生物标志物，用于准确预测患者预后，并作为ICC治疗的靶点。
