Sun Lei, Chen Xuzhuo, Mu Haizhang, Xu Yin, Chen Ruiguo, Xia Rong, Xia Lunguo, Zhang Shanyong
Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University and Shandong Key Laboratory of Oral Tissue Regeneration and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.
Department of Stomatology, The Second Affiliated Hospital of Anhui Medical University, Hefei, China.
Front Bioeng Biotechnol. 2022 Feb 24;10:841591. doi: 10.3389/fbioe.2022.841591. eCollection 2022.
Nest-like nanofiber structures have potential applications in surface modifications of titanium implants. In this study, nest-like nanofiber structures were prepared on a titanium surface at room temperature and pressure by using the nanobowl template-assisted method combined with alkali etching. The characterization and biocompatibility of this material were analyzed by cellular adhesion, death, CCK-8, ALP, and RT-PCR assays and osseointegration was evaluated by micro-CT and fluorescent labeling . The results showed that this nest-like nanofiber structure has a firmer and asperate surface than nanotubes, which leads to better cellular adhesion, proliferation, and differentiation capacity. In a beagle alveolar bone implant model, the nest-like nanofiber structure showed a better osseointegration capacity. In conclusion, this nest-like nanofiber structure has potential applications in dental implantology.
巢状纳米纤维结构在钛植入物表面改性方面具有潜在应用。在本研究中，通过纳米碗模板辅助法结合碱蚀刻在室温常压下于钛表面制备了巢状纳米纤维结构。通过细胞黏附、死亡、CCK - 8、碱性磷酸酶（ALP）和逆转录聚合酶链反应（RT - PCR）分析对该材料的表征和生物相容性进行了分析，并通过微型计算机断层扫描（micro - CT）和荧光标记评估了骨整合情况。结果表明，这种巢状纳米纤维结构比纳米管具有更坚硬且粗糙的表面，这导致其具有更好的细胞黏附、增殖和分化能力。在比格犬牙槽骨植入模型中，巢状纳米纤维结构显示出更好的骨整合能力。总之，这种巢状纳米纤维结构在牙种植学中具有潜在应用。
