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评估单眼剥夺对幼年小鼠视觉诱发电位、行为及视觉可塑性的影响。

Assessing the impact of monocular deprivation on visual evoked potentials, behavior, and visual plasticity in juvenile mice.

作者信息

Ding Suzhen, Yang Chunxian, Zhu Hefei, Li Shaomin, Li Lan

机构信息

Department of Ophthalmology, the First People's Hospital of Kunming, Kunming, China.

Key Laboratory of Animal Models and Human Disease Mechanisms, Lab of Learning and Memory, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.

出版信息

Ann Transl Med. 2022 Feb;10(3):141. doi: 10.21037/atm-21-6723.

DOI:10.21037/atm-21-6723
PMID:35284534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8904994/
Abstract

BACKGROUND

The physiological mechanisms which underlie amblyopia are predicted using animal models which assess the impact of amblyogenic factors on visual function. This study used monocular-deprived mice as an amblyopic model to assess visual function by flash visual evoked potentials (fVEP), behavioral assessment, and visual plasticity.

METHODS

A total of 294 C57BL/6J mice (both genders) were used in this study. The mice were divided into the normal control (NC) group and monocular deprivation (MD) group. After mice were anesthetized with pentobarbital, fVEP was recorded. Long-term potentiation (LTP) was recorded from primary visual cortex slices. Behavioral assessment of visual function was performed using a visual water trapezoidal-shaped pool with a release chute, a hidden platform, and a middle divider.

RESULTS

All fVEP results showed that N1 waves and P2 waves were repeatable and N1-P2 amplitude was the most stable indicator. The amplitude of N1-P2 of MD eyes was significantly lower than that of non-deprived eyes or NC eyes. LTP failed to be induced in the visual cortex V1 area corresponding to deprived eyes in the MD group but could be induced successfully in the visual cortex V1 area corresponding to non-deprived eyes in the MD group. Behavioral vision testing also showed a longer time to reach the platform in MD mice compared to NC mice. The correlation coefficient suggested that LTP is the better indicator for visual impairment.

CONCLUSIONS

The fVEP can be utilized as an index of amblyopic changes in mice, which correlates well with behavioral results.

摘要

背景

利用动物模型预测弱视潜在的生理机制，这些模型评估致弱视因素对视觉功能的影响。本研究使用单眼剥夺小鼠作为弱视模型，通过闪光视觉诱发电位（fVEP）、行为评估和视觉可塑性来评估视觉功能。

方法

本研究共使用294只C57BL/6J小鼠（雌雄均有）。将小鼠分为正常对照组（NC）和单眼剥夺组（MD）。用戊巴比妥麻醉小鼠后，记录fVEP。从初级视觉皮层切片记录长时程增强（LTP）。使用带有释放斜槽、隐藏平台和中间分隔物的视觉水梯形池对视觉功能进行行为评估。

结果

所有fVEP结果显示，N1波和P2波具有可重复性，N1 - P2波幅是最稳定的指标。MD组剥夺眼的N1 - P2波幅显著低于未剥夺眼或NC组眼。MD组中与剥夺眼对应的视觉皮层V1区未能诱导出LTP，但在MD组中与未剥夺眼对应的视觉皮层V1区可成功诱导出LTP。行为视觉测试还显示，与NC小鼠相比，MD小鼠到达平台的时间更长。相关系数表明LTP是视觉损伤的更好指标。

结论

fVEP可作为小鼠弱视变化的指标，与行为结果相关性良好。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/6bf9e007a9fe/atm-10-03-141-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/7261d8fbf23a/atm-10-03-141-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/7d15c95ae239/atm-10-03-141-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/9501fbe6cebe/atm-10-03-141-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/1ff6451c4f3d/atm-10-03-141-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/0acac442a498/atm-10-03-141-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/6bf9e007a9fe/atm-10-03-141-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/7261d8fbf23a/atm-10-03-141-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/7d15c95ae239/atm-10-03-141-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/9501fbe6cebe/atm-10-03-141-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/1ff6451c4f3d/atm-10-03-141-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/0acac442a498/atm-10-03-141-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7364/8904994/6bf9e007a9fe/atm-10-03-141-f6.jpg

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