Stroehlein Andreas J, Korhonen Pasi K, Rollinson David, Stothard J Russell, Hall Ross S, Gasser Robin B, Young Neil D
Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, 3010, Australia.
Natural History Museum, London, UK.
Curr Res Parasitol Vector Borne Dis. 2021 Feb 10;1:100015. doi: 10.1016/j.crpvbd.2021.100015. eCollection 2021.
Despite advances in high-throughput sequencing and bioinformatics, molecular investigations of snail intermediate hosts that transmit parasitic trematodes are scant. Here, we report the first transcriptome for - a key intermediate host of - a blood fluke that causes urogenital schistosomiasis in humans. We assembled this transcriptome from short- and long-read RNA-sequence data. From this transcriptome, we predicted 12,998 proteins, 58% of which had orthologs in an intermediate host of - a blood fluke that causes hepato-intestinal schistosomiasis. We predicted that select protein groups are involved in signal transduction, cell growth and death, the immune system, environmental adaptation and/or the excretory/secretory system, suggesting roles in immune responses, pathogen defence and/or parasite-host interactions. The transcriptome of provides a useful resource to underpin future molecular investigations of this and related snail species, and its interactions with pathogens including The present resource should enable comparative investigations of other molluscan hosts of socioeconomically important parasites in the future.
尽管高通量测序和生物信息学取得了进展，但对传播寄生吸虫的蜗牛中间宿主的分子研究却很少。在这里，我们报告了——一种导致人类泌尿生殖系统血吸虫病的血吸虫的关键中间宿主——的首个转录组。我们从短读长和长读长RNA序列数据中组装了这个转录组。从这个转录组中，我们预测了12998种蛋白质，其中58%在——一种导致肝肠血吸虫病的血吸虫的中间宿主——中有直系同源物。我们预测特定的蛋白质组参与信号转导、细胞生长和死亡、免疫系统、环境适应和/或排泄/分泌系统，表明它们在免疫反应、病原体防御和/或寄生虫-宿主相互作用中发挥作用。的转录组为支持对该蜗牛物种及相关蜗牛物种的未来分子研究，以及其与包括在内的病原体的相互作用提供了有用的资源。目前的资源应能在未来对具有社会经济重要性的寄生虫的其他软体动物宿主进行比较研究。
