Zhu Tong, Yang Dan, Liu Shan-Shan, Teng Fei, Zhu Jing-Jing, Zhang Yong-Xin, Xu Guo-Bing, Liu Shou-Jin, Wang Zhi-Min, Chen Liang-Mian, Gao Hui-Min
School of Pharmary, Anhui University of Chinese Medicine Hefei 230012, China National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2022 Feb;47(4):980-987. doi: 10.19540/j.cnki.cjcmm.20211102.201.
The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MSE) technology was employed to compare the chemical components between the aerial and underground parts of Coptis chinensis samples from different batches. According to the retention time, molecular ion peak, and LC-MSE fragment information of the reference substances and available literature, we identified a total of 40 components. Thirty-three and 31 compounds were respectively identified in the underground part(taproots) and the aerial part(stems and leaves) of C. chinensis. Among them, 24 compounds, including alkaloids(e.g., berberine and jatrorrhizine) and phenolic acids(e.g., chlorogenic acid, quinic acid, and tanshinol), were common in the two parts. In addition, differential components were also identified, such as magnoline glucoside in the underground part and(±) lariciresionol-4-β-D-glucopyranoside in the aerial part. The analysis of fragmentation pathways based on spectra of reference substances indicated the differences among samples of different batches. Furthermore, we performed the principal component analysis(PCA) for the peak areas of C. chinensis in different batches. The results showed that the underground part and the aerial part were clearly clustered into two groups, indicating that the chemical components contained in the two parts were different. Furthermore, the results of partial least squares discriminant analysis(PLS-DA) identified 31 differential compounds(VIP value>1) between the underground part and the aerial part, mainly including alkaloids, phenolic acids, lignans, and flavonoids. This study proves that C. chinensis possesses great development potential with multiple available compounds in stems and leaves. Moreover, it sheds light on for the development and utilization of non-medicinal organs of C. chinensis and other Chinese medicinal herbs.
采用超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MSE）技术比较不同批次黄连样品地上部分和地下部分的化学成分。根据对照品的保留时间、分子离子峰以及LC-MSE碎片信息并结合已有文献，共鉴定出40种成分。在黄连的地下部分（主根）和地上部分（茎和叶）分别鉴定出33种和31种化合物。其中，24种化合物在两部分中都存在，包括生物碱（如小檗碱和药根碱）和酚酸（如绿原酸、奎宁酸和丹参素）。此外，还鉴定出了差异成分，如地下部分的木兰苷和地上部分的（±）落叶松脂醇-4-β-D-葡萄糖苷。基于对照品光谱的碎片途径分析表明不同批次样品之间存在差异。此外，我们对不同批次黄连的峰面积进行了主成分分析（PCA）。结果表明，地下部分和地上部分明显聚为两组，表明两部分所含化学成分不同。此外，偏最小二乘判别分析（PLS-DA）结果确定了地下部分和地上部分之间31种差异化合物（VIP值>1），主要包括生物碱、酚酸、木脂素和黄酮类化合物。本研究证明黄连的茎和叶中含有多种可利用的化合物，具有很大的开发潜力。此外，该研究为黄连及其他中药材非药用器官的开发利用提供了思路。
