Department of Dermato-Venereology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
Epigenomics. 2022 Apr;14(8):431-449. doi: 10.2217/epi-2022-0016. Epub 2022 Mar 14.
To explore advanced glycation end products (AGEs)-induced mA modification in fibroblasts and its potential role in photoaging. We studied mA modification in AGEs-bovine serum albumin-treated fibroblasts with mA-mRNA & lncRNA epitranscriptomic microarray and bioinformatics analysis. The mA modification level was also investigated in skin samples. mA methylation microarray analysis revealed mA modification profiles in AGEs-treated fibroblasts. Gene ontology, Kyoto Encyclopedia of Genes and Genomes, protein-protein interaction and competing endogenous RNA network analysis indicated that the genes of differentially methylated mRNAs and lncRNAs were mainly related to inflammation processes. We also found that AGEs-bovine serum albumin dose-dependently increased the mA level and expression in both fibroblasts and sun-exposed skin. Our study provided novel information regarding alterations of mA modifications in AGEs-induced dermal fibroblasts and potential targets for treatment of photoaging.
为了探究糖基化终产物（AGEs）诱导的成纤维细胞 mA 修饰及其在光老化中的潜在作用。我们通过 mA-mRNA 和 lncRNA 转录组修饰微阵列和生物信息学分析研究了 AGEs-牛血清白蛋白处理的成纤维细胞中的 mA 修饰。我们还研究了皮肤样本中的 mA 修饰水平。mA 甲基化微阵列分析揭示了 AGEs 处理的成纤维细胞中的 mA 修饰图谱。基因本体论、京都基因与基因组百科全书、蛋白质-蛋白质相互作用和竞争性内源性 RNA 网络分析表明，差异甲基化 mRNAs 和 lncRNAs 的基因主要与炎症过程有关。我们还发现 AGEs-牛血清白蛋白以剂量依赖的方式增加了成纤维细胞和暴露于阳光的皮肤中的 mA 水平和表达。我们的研究为 AGEs 诱导的皮肤成纤维细胞中 mA 修饰的改变及其作为光老化治疗的潜在靶点提供了新的信息。
