Hossain Abul, Dave Deepika, Shahidi Fereidoon
Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Marine Bioprocessing Facility, Centre of Aquaculture and Seafood Development, Marine Institute, Memorial University, St. John's, NL A1B 3X9, Canada.
J Agric Food Chem. 2022 Mar 23;70(11):3489-3501. doi: 10.1021/acs.jafc.2c00140. Epub 2022 Mar 14.
Sea cucumber contains a wide range of bioactive compounds, including phenolics. This study investigated the free, esterified, and insoluble-bound phenolics of sea cucumber body wall as affected by high-pressure processing (HPP) pretreatment. Sea cucumber body wall was subjected to HPP (200, 400, and 600 MPa for 5, 10, and 15 min), followed by the extraction of phenolics. The contents of total phenolics and antioxidant activity were monitored. Compared to untreated samples, those treated with HPP exhibited significantly higher total phenolics, flavonoids, and antioxidant activities. Treatment of 600 MPa for 10 min offered the optimal results. The highest amount of phenolics was observed in the free phenolic fraction, followed by esterified and insoluble-bound phenolic fractions. Moreover, phenolic extracts showed inhibitory effects against cupric ion-induced low-density lipoprotein (LDL)-cholesterol oxidation, peroxyl and hydroxyl radical-induced DNA scission, α-glucosidase activity, and formation of advanced glycation end products (AGEs). Ultra-high-performance liquid chromatography equipped with a quadrupole time of fight and mass spectrometer (UHPLC-QTOF-MS/MS) identified 20 phenolic compounds, mainly phenolic acids and flavonoids, from the body wall of this species for the first time. Thus, sea cucumber may lead to the production of a multitude of value-added products.
海参含有多种生物活性化合物，包括酚类物质。本研究调查了高压处理（HPP）预处理对海参体壁中游离、酯化和不溶性结合酚类物质的影响。对海参体壁进行HPP处理（200、400和600兆帕，处理5、10和15分钟），然后提取酚类物质。监测总酚含量和抗氧化活性。与未处理的样品相比，经HPP处理的样品总酚、黄酮类化合物和抗氧化活性显著更高。600兆帕处理10分钟效果最佳。游离酚类组分中酚类物质含量最高，其次是酯化和不溶性结合酚类组分。此外，酚类提取物对铜离子诱导的低密度脂蛋白（LDL）胆固醇氧化、过氧自由基和羟基自由基诱导的DNA断裂、α-葡萄糖苷酶活性以及晚期糖基化终产物（AGEs）的形成具有抑制作用。配备四极杆飞行时间质谱仪的超高效液相色谱（UHPLC-QTOF-MS/MS）首次从该物种的体壁中鉴定出20种酚类化合物，主要为酚酸和黄酮类化合物。因此，海参可能会带来多种增值产品的生产。
