Department of Electronics and Communication Engineering, Koneru Lakshmaiah Education Foundation (Deemed to Be University), Vaddeswaram, Guntur, 522502, India.
Department of Electronics and Telecommunication Engineering, Indian Institute of Engineering Science and Technology, Shibpur, West Bengal 711103, India.
Talanta. 2022 Jun 1;243:123368. doi: 10.1016/j.talanta.2022.123368. Epub 2022 Mar 10.
Solid-state nanopore has the ability to detect proteins at a single-molecule level with its high sensitivity, high-throughput, and low cost. Improvements in fabrication, functionalization, and characterization of solid-state nanopores keep evolving. Various analytical methods targeted towards diagnostic applications using nanopore-based devices are appearing. This review article provides an overview of recent progress in the field of solid-state and biological nanopores for protein detection in a complex analyte. The advantages and challenges involved in nanopore sensing have been discussed. Further, the review surpasses the steady-state resistive pulse techniques of sensing and incorporate transient variations in the nanopore conductance. Application of the power spectral density of these fluctuations toward sensing has been highlighted with importance on reducing the detection limit in a complex environment. Lastly, the current problems and future perspectives have been discussed with a perspective to increase nanopores performance towards diagnostic applications in complex medium.
固态纳米孔具有高灵敏度、高通量和低成本的特点，能够在单分子水平上检测蛋白质。固态纳米孔的制造、功能化和表征技术不断改进。基于纳米孔的设备针对诊断应用的各种分析方法正在出现。本文综述了近年来在复杂分析物中用于蛋白质检测的固态和生物纳米孔领域的最新进展。讨论了纳米孔传感的优点和挑战。此外，本综述超越了稳态电阻脉冲技术的传感，并结合了纳米孔电导的瞬态变化。强调了这些波动的功率谱密度在传感中的应用，以降低复杂环境中的检测限。最后，讨论了当前的问题和未来的展望，以期提高纳米孔在复杂介质中诊断应用的性能。
