Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China.
Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin, PR China.
Drug Chem Toxicol. 2023 May;46(3):451-461. doi: 10.1080/01480545.2022.2050749. Epub 2022 Mar 15.
(Sieb. et Zucc.) Maxim., a traditional medicinal plant in Asia, is widely used in clinical settings but its safety is unclear. This study investigated the sub-chronic toxicity of aqueous extract to rats with a 13-week daily intragastric administration of 7.5, 15, or 30 g/kg. Nine constituents of the aqueous extract were identified by ultra-performance liquid chromatography (UPLC). Organ weights, organ coefficients, serum biochemistry parameters, histopathology, and metabolomic analysis were performed. In female rats, treatment increased the liver, thymus, and adrenal gland coefficients ( < 0.05). Liver, pancreas, and adrenal gland injury were observed. The levels of six metabolites were altered by the treatment ( < 0.05). In male rats, treatment altered liver, heart, and thymus coefficients ( < 0.05) and liver, adrenal gland, and heart injury were observed. The levels of 11 metabolites were altered ( < 0.05). The no-observed-adverse-effect level was not determined but would be below 7.5 g/kg in rats treated for 13 weeks. In female rats, may injure the liver and pancreas and dysregulate the biosynthesis of phenylalanine, tyrosine, tryptophan, valine, leucine, and isoleucine and the metabolism of phenylalanine. In male rats, the extract may injure the liver and adrenal gland and dysregulate the biosynthesis of valine, leucine, and isoleucine and the metabolism of pyruvate.
（Siebold et Zucc.）Maxim. 是亚洲一种传统药用植物，广泛用于临床，但安全性尚不清楚。本研究通过为期 13 周的每日胃内给予 7.5、15 或 30g/kg 的水提物，对大鼠进行了亚慢性毒性研究。通过超高效液相色谱法（UPLC）鉴定了水提物的 9 种成分。进行了器官重量、器官系数、血清生化参数、组织病理学和代谢组学分析。在雌性大鼠中，治疗增加了肝脏、胸腺和肾上腺系数（<0.05）。观察到肝脏、胰腺和肾上腺损伤。有 6 种代谢物的水平因治疗而改变（<0.05）。在雄性大鼠中，治疗改变了肝脏、心脏和胸腺系数（<0.05）并观察到肝脏、肾上腺和心脏损伤。有 11 种代谢物的水平发生了改变（<0.05）。未观察到不良效应水平，但在 13 周治疗的大鼠中，该水平将低于 7.5g/kg。在雌性大鼠中，可能会损伤肝脏和胰腺，并扰乱苯丙氨酸、酪氨酸、色氨酸、缬氨酸、亮氨酸和异亮氨酸的生物合成以及苯丙氨酸的代谢。在雄性大鼠中，提取物可能会损伤肝脏和肾上腺，并扰乱缬氨酸、亮氨酸和异亮氨酸的生物合成以及丙酮酸的代谢。
