Gamas P, Burger A C, Churchward G, Caro L, Galas D, Chandler M
Mol Gen Genet. 1986 Jul;204(1):85-9. doi: 10.1007/BF00330192.
We have shown that the plasmid pSC101 is unable to be maintained in strains of E. coli carrying deletions in the genes himA and hip which specify the pleitropic heterodimeric DNA binding protein, IHF. We show that this effect is not due to a modulation of the expression of the pSC101 RepA protein, required for replication of the plasmid. Inspection of the DNA sequence of the essential replication region of pSC101 reveals the presence of a site, located between the DnaA binding-site and that of RepA, which shows extensive homology with the consensus IHF binding site. The proximity of the sites suggests that these three proteins, IHF, DnaA, and RepA may interact in generating a specific DNA structure required for initiation of pSC101 replication.
我们已经表明，质粒pSC101无法在携带himA和hip基因缺失的大肠杆菌菌株中维持，这两个基因编码多效性异源二聚体DNA结合蛋白IHF。我们表明，这种效应不是由于质粒复制所需的pSC101 RepA蛋白表达的调节。对pSC101必需复制区域的DNA序列检查发现，在DnaA结合位点和RepA结合位点之间存在一个位点，该位点与共有IHF结合位点具有广泛的同源性。这些位点的接近表明，这三种蛋白质，即IHF、DnaA和RepA，可能在产生pSC101复制起始所需的特定DNA结构中相互作用。
