Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-Sen University, No. 58 Zhongshan 2nd Road, Guangzhou, 510080, Guangdong, China.
Institute of Respiratory Diseases of Sun Yat-Sen University, No. 58 Zhongshan 2nd Road, Guangzhou, 510080, Guangdong, China.
BMC Pulm Med. 2022 Mar 14;22(1):86. doi: 10.1186/s12890-022-01887-2.
Baculoviral IAP repeat-containing 3 (BIRC3) which encodes a member of the IAP family of proteins upregulated in the asthma expression profile dataset. However, there was few research on studying the clinical implication of BIRC3 in asthma.
To validate BIRC3 expression and its clinical implications in induced sputum of asthma.
Based on the GSE76262 (118 asthma cases and 21 healthy controls) dataset, differentially expressed genes were screened using R software. Subsequently, BIRC3 mRNA and protein were clinically verified in induced sputum samples through quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). Besides, the correlations between BIRC3 expression and asthmatic eosinophilic/allergic inflammation indicators (FeNO, IgE, and EOS%), pulmonary function (FEV, FEV% pred, FVC% pred, and FEV/FVC), and inflammatory cytokines (IL-4, IL-5, IL-13, IL-25, IL-10, IL-33, and TSLP) were analyzed. Finally, BIRC3 mRNA was detected in human primary bronchial epithelial cells stimulated by cytokines (IL-4 or IL-13).
BIRC3 was screened as a candidate gene in the GSE76262, which was highly expressed in asthma. Highly expressed BIRC3 was positively correlated with eosinophilic and allergic indicators, including FeNO, blood eosinophil, and serum IgE. Moreover, BIRC3 protein was positively associated with inflammation cytokines, like IL-4, IL-5, IL-13, IL-25, IL-10, IL-33, and TSLP, while negatively correlated with FEV1, FEV1%pred, FVC% pred, and FEV1/FVC. Furthermore, the expression of BIRC3 could be induced in primary bronchial epithelial cells treated by cytokines IL-4 or IL-13.
BIRC3 significantly increased in induced sputum of asthma and positively correlated with airway eosinophilic and peripheral blood allergic inflammation, type 2 cytokines, and airway obstruction. Increased BIRC3 might be involved in the pathogenesis of asthma by affecting the eosinophilic and allergic inflammation.
杆状病毒 IAP 重复序列包含 3（BIRC3），它编码哮喘表达谱数据集中上调的 IAP 家族蛋白的成员。然而，关于 BIRC3 在哮喘中的临床意义的研究很少。
验证 BIRC3 在哮喘诱导痰中的表达及其临床意义。
基于 GSE76262（118 例哮喘病例和 21 例健康对照）数据集，使用 R 软件筛选差异表达基因。随后，通过定量实时聚合酶链反应（qRT-PCR）和酶联免疫吸附试验（ELISA）在诱导痰样本中临床验证 BIRC3mRNA 和蛋白。此外，还分析了 BIRC3 表达与哮喘嗜酸性粒细胞/过敏炎症指标（FeNO、IgE 和 EOS%）、肺功能（FEV、FEV%pred、FVC%pred 和 FEV/FVC）和炎症细胞因子（IL-4、IL-5、IL-13、IL-25、IL-10、IL-33 和 TSLP）之间的相关性。最后，检测细胞因子（IL-4 或 IL-13）刺激的人原代支气管上皮细胞中 BIRC3mRNA 的表达。
BIRC3 作为 GSE76262 中的候选基因被筛选出来，在哮喘中表达较高。高表达的 BIRC3 与嗜酸性粒细胞和过敏指标呈正相关，包括 FeNO、血嗜酸性粒细胞和血清 IgE。此外，BIRC3 蛋白与炎症细胞因子如 IL-4、IL-5、IL-13、IL-25、IL-10、IL-33 和 TSLP 呈正相关，与 FEV1、FEV1%pred、FVC%pred 和 FEV1/FVC 呈负相关。此外，细胞因子 IL-4 或 IL-13 处理的原代支气管上皮细胞中可诱导 BIRC3 表达。
BIRC3 在哮喘诱导痰中显著增加，与气道嗜酸性粒细胞和外周血过敏炎症、2 型细胞因子和气道阻塞呈正相关。BIRC3 的增加可能通过影响嗜酸性粒细胞和过敏炎症参与哮喘的发病机制。
