Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Guangdong, China.
Institute of Respiratory Diseases of Sun Yat-Sen University, Guangdong, China.
Int Arch Allergy Immunol. 2023;184(11):1135-1142. doi: 10.1159/000531073. Epub 2023 Aug 16.
Asthma is a chronic disease that affects populations worldwide. The purpose of this study was to investigate the expression of TCN1 in sputum and its correlation with inflammation and lung function in asthma.
We recruited 141 subjects, detected TCN1 mRNA level by quantitative reverse transcription polymerase chain reaction, detected TCN1 protein expression by Western blot, detected TCN1 protein level by enzyme-linked immunosorbent assay, and analyzed the correlation between TCN1 and fraction of exhaled nitric oxide (FeNO), IgE, EOS%, lung functions, and some Th2 cytokines. The diagnostic value of TCN1 was evaluated by receiver operating characteristics curve. The expression of TCN1 was further confirmed by human bronchial epithelial cell in vitro.
Compared with the health group, the expression of TCN1 in induced sputum cells increased in asthma group and was correlated with FeNO, IgE, and EOS%. TCN1 level was also elevated in the induced sputum supernatant of asthma patients. The protein level of TCN1 in induced sputum supernatant was correlated with FeNO, IgE and PC-20, forced expiratory volume in the first second (FEV1)%pred, FEV1/FVC, and some cytokines (IL-4, IL-5, IL-10, IL-13, MUC5AC). TCN1 was also differentially expressed in patients with different severity of asthma. Four weeks after ICS treatment, the expression of TCN1 in induced sputum supernatant increased. In vitro, the protein level of TCN1 in human bronchial epithelial cells' supernatant increased after stimulated with IL-4 and IL-13.
The expression of TCN1 was increased in asthma patients' sputum, and was positively correlated with some inflammatory markers, negatively correlated with lung function. TCN1 may be used as a potential biomarker for the diagnosis and treatment of asthma.
哮喘是一种影响全球人群的慢性疾病。本研究旨在探讨 TCN1 在痰液中的表达及其与哮喘炎症和肺功能的关系。
我们招募了 141 名受试者，通过定量逆转录聚合酶链反应检测 TCN1 mRNA 水平，通过 Western blot 检测 TCN1 蛋白表达，通过酶联免疫吸附试验检测 TCN1 蛋白水平，并分析 TCN1 与呼出气一氧化氮（FeNO）、IgE、EOS%、肺功能和一些 Th2 细胞因子之间的相关性。通过受试者工作特征曲线评估 TCN1 的诊断价值。通过体外人支气管上皮细胞进一步证实 TCN1 的表达。
与健康组相比，哮喘组诱导痰细胞中 TCN1 的表达增加，且与 FeNO、IgE 和 EOS%相关。哮喘患者诱导痰上清液中 TCN1 水平也升高。诱导痰上清液中 TCN1 蛋白水平与 FeNO、IgE 和 PC-20、第一秒用力呼气容积（FEV1）%pred、FEV1/FVC 以及一些细胞因子（IL-4、IL-5、IL-10、IL-13、MUC5AC）相关。TCN1 在不同严重程度的哮喘患者中也有差异表达。ICS 治疗 4 周后，诱导痰上清液中 TCN1 的表达增加。体外，IL-4 和 IL-13 刺激后，人支气管上皮细胞上清液中 TCN1 蛋白水平增加。
哮喘患者痰液中 TCN1 的表达增加，与一些炎症标志物呈正相关，与肺功能呈负相关。TCN1 可能作为哮喘诊断和治疗的潜在生物标志物。
