诱导痰中 SERPINB10 表达增加与哮喘气道 2 型炎症相关。
Increased SERPINB10 Expression in Induced Sputum Was Associated with Airway Type 2 Inflammation in Asthma.
机构信息
Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China,
Department of Pulmonary and Critical Care Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
出版信息
Int Arch Allergy Immunol. 2022;183(9):946-952. doi: 10.1159/000524613. Epub 2022 May 6.
BACKGROUND
Asthma is a common chronic airway inflammation in the world. The aim of this study was to investigate the expression of SERPINB10 in induced sputum and its correlation with airway type 2 inflammation in asthma.
METHODS
We recruited 90 subjects and detected SERPINB10 levels in induced sputum by ELISA and analyzed the correlation between SERPINB10 expression levels and FeNO, eosinophils in peripheral blood, lung function, and Th2 cytokines (IL-4, IL-5, and IL-13).
RESULTS
The levels of SERPINB10 in induced sputum in asthmatic patients were higher than healthy controls. SERPINB10 levels in induced sputum were positively correlated with FeNO (r = 0.4620, p < 0.0001) and eosinophils in peripheral blood (r = 0.2500, p = 0.0218) and negatively correlated with FEV1 (%predicted) (r = -0.4161, p < 0.0001) and FEV1/FVC% (r = -0.4383, p < 0.0001). SERPINB10 levels were correlated with Th2 cytokines IL-4 (r = 0.6274, p < 0.0001), IL-5 (r = 0.5166, p < 0.0001), and IL-13 (r = 0.5212, p = 0.0003) in asthma.
CONCLUSIONS
SERPINB10 levels in induced sputum of asthmatic patients were significantly increased and correlated with asthmatic airway type 2 inflammation. Induced sputum SERPINB10 may be a signature protein for type 2 high asthma and may be a potential target for airway eosinophilic inflammation in asthma.
背景
哮喘是世界范围内常见的慢性气道炎症。本研究旨在探讨诱导痰中 SERPINB10 的表达及其与哮喘气道 2 型炎症的相关性。
方法
我们招募了 90 名受试者，通过 ELISA 检测诱导痰中 SERPINB10 的水平，并分析 SERPINB10 表达水平与 FeNO、外周血嗜酸性粒细胞、肺功能和 Th2 细胞因子（IL-4、IL-5 和 IL-13）之间的相关性。
结果
哮喘患者诱导痰中 SERPINB10 的水平高于健康对照组。诱导痰中 SERPINB10 的水平与 FeNO 呈正相关（r = 0.4620，p < 0.0001），与外周血嗜酸性粒细胞呈正相关（r = 0.2500，p = 0.0218），与 FEV1（%预计值）呈负相关（r = -0.4161，p < 0.0001），与 FEV1/FVC%呈负相关（r = -0.4383，p < 0.0001）。SERPINB10 水平与 Th2 细胞因子 IL-4（r = 0.6274，p < 0.0001）、IL-5（r = 0.5166，p < 0.0001）和 IL-13（r = 0.5212，p = 0.0003）在哮喘中呈正相关。
结论
哮喘患者诱导痰中 SERPINB10 的水平显著升高，并与哮喘气道 2 型炎症相关。诱导痰 SERPINB10 可能是 2 型高气道炎症的特征性蛋白，可能是哮喘气道嗜酸性粒细胞炎症的潜在靶点。
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