Parasitology, Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, PO box 5003, Ås, 1432, Norway.
Parasitology, Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, PO box 5003, Ås, 1432, Norway.
Food Microbiol. 2022 Jun;104:103980. doi: 10.1016/j.fm.2022.103980. Epub 2022 Jan 12.
The risk of foodborne parasite infection linked to the consumption of contaminated fresh produce has long been known. However, despite epidemiological links between the outbreaks and contaminated berries, few studies have assessed the magnitude of parasite contamination on fresh produce sold in Europe. The present study was aimed to address the knowledge gap on parasite contamination of berries sold in Norway. Samples of blueberries, strawberries, and raspberries were analysed by multiplex qPCR for detection of Echinococcus multilocularis, Toxoplasma gondii, and Cyclospora cayetanensis. In addition, a simplex qPCR method was employed for detecting contamination of the berries with Cryptosporidium spp. A total of 820 samples of berries, each of around 30 g (274 samples of blueberries, 276 samples of raspberries, and 270 samples of strawberries), were analysed. We found an overall occurrence of 2.9%, 6.6%, and 8.3% for T. gondii, C. cayetanensis, and Cryptosporidium spp., respectively, whereas E. multilocularis was not detected from any of the samples investigated. Strawberries and raspberries were most often contaminated with Cryptosporidium spp., whereas blueberries were contaminated mostly with C. cayetanensis. Detection of parasite contaminants on fresh berries indicates the need for a system to ensure the parasitological safety of fresh berries.
食用受污染的新鲜农产品而导致食源性寄生虫感染的风险早已为人所知。然而，尽管与污染浆果有关的爆发之间存在流行病学联系，但很少有研究评估欧洲销售的新鲜农产品上寄生虫污染的程度。本研究旨在填补有关挪威销售的浆果寄生虫污染的知识空白。通过多重 qPCR 分析蓝莓、草莓和覆盆子样本，以检测多房棘球绦虫、刚地弓形虫和环孢子虫。此外，还采用 simplex qPCR 方法检测浆果是否受到隐孢子虫属的污染。总共分析了 820 份每份约 30 克的浆果样本（274 份蓝莓样本、276 份覆盆子样本和 270 份草莓样本）。我们发现 T. gondii、C. cayetanensis 和 Cryptosporidium spp. 的总体发生率分别为 2.9%、6.6%和 8.3%，而从调查的任何样本中均未检测到多房棘球绦虫。草莓和覆盆子最常受到隐孢子虫属的污染，而蓝莓则主要受到环孢子虫的污染。在新鲜浆果上检测到寄生虫污染物表明需要建立一个系统来确保新鲜浆果的寄生虫安全性。
