Almeria Sonia, Chacin-Bonilla Leonor, Maloney Jenny G, Santin Monica
Center for Food Safety and Nutrition (CFSAN), Department of Health and Human Services, Food and Drug Administration, Office of Applied Research and Safety Assessment (OARSA), Division of Virulence Assessment, Laurel, MD 20708, USA.
Institute of Clinical Research, University of Zulia, Maracaibo 4001-A, Venezuela.
Microorganisms. 2023 Aug 28;11(9):2171. doi: 10.3390/microorganisms11092171.
infections are prevalent worldwide, and the parasite has become a major public health and food safety concern. Although important efforts have been dedicated to advance toward preventing and reducing incidences of cyclosporiasis, there are still several knowledge gaps that hamper the implementation of effective measures to prevent the contamination of produce and water with oocysts. Some of these data gaps can be attributed to the fact that access to oocysts is a limiting factor in research. There are no animal models or in vivo or in vitro culture systems to propagate the oocysts needed to facilitate research. Thus, researchers must rely upon limited supplies of oocysts obtained from naturally infected human patients considerably restricting what can be learnt about this parasite. Despite the limited supply of oocysts, several important advances have happened in the past 3 years. Great progress has been made in the field in the areas of molecular characterization of strains and species, generation of genomes, and development of novel detection methods. This comprehensive perspective summarizes research published from 2020 to 2023 and evaluates what we have learnt and identifies those aspects in which further research is needed.
感染在全球范围内普遍存在，这种寄生虫已成为主要的公共卫生和食品安全问题。尽管人们已付出重要努力来推动预防和减少环孢子虫病的发病率，但仍存在一些知识空白，阻碍了采取有效措施防止农产品和水源被卵囊污染。其中一些数据空白可归因于获取卵囊是研究中的一个限制因素。目前没有动物模型或体内或体外培养系统来繁殖开展研究所需的卵囊。因此，研究人员必须依赖从自然感染人类患者获取的有限卵囊供应，这极大地限制了对这种寄生虫的了解。尽管卵囊供应有限，但在过去3年中仍取得了一些重要进展。在菌株和物种的分子特征分析、基因组生成以及新型检测方法开发等领域都取得了重大进展。这篇综述总结了2020年至2023年发表的研究，并评估了我们所了解的情况，确定了需要进一步研究的方面。
