College of Forestry, Northwest A&F University, Yangling 712100, China.
Beijing Forestry University, Beijing Key Lab Lignocellulosic Chemistry, Beijing 100083, China.
Carbohydr Polym. 2022 Jun 1;285:119217. doi: 10.1016/j.carbpol.2022.119217. Epub 2022 Feb 5.
Xylan extraction by alkali is usually a prerequisite for traditional xylooligosaccharide (XOS) production from corncob. In this study, to avoid xylan isolation before XOS production, corncob was delignified by hydrogen peroxide-acetic acid (HPAA), followed by a two-step enzymatic hydrolysis for XOS and monosaccharide production. High lignin removal (55.5%-82.6%) was observed upon pretreatment with 75%-100% HPAA and using 50-100 mM HSO as a catalyst. A high XOS yield of 27.8% was obtained from 75% HPAA-pretreated corncob with 75 mM HSO, and the xylose/XOS ratio was only 0.09, indicating that the purity of XOS was relatively high. The highest glucose yield (79.1%) was obtained from 100% HPAA-pretreated corncob with 50 mM HSO. Finally, 58.3 g of XOS, 186.9 g of glucose, and 56.4 g of xylose were obtained from 1 kg of corncob. This study provides a promising approach for XOS and monosaccharide production from corncob without xylan isolation.
碱法提取木聚糖通常是玉米芯传统低聚木糖（XOS）生产的前提条件。在这项研究中，为避免在 XOS 生产前分离木聚糖，采用过氧化氢-乙酸（HPAA）对玉米芯进行脱木质素处理，然后进行两步酶解生产 XOS 和单糖。用 75%至 100%的 HPAA 和 50-100mM 的 HSO 作为催化剂预处理时，观察到高木质素去除率（55.5%-82.6%）。用 75mM 的 HSO 从 75% HPAA 预处理的玉米芯中获得了 27.8%的高 XOS 产率，木糖/XOS 比仅为 0.09，表明 XOS 的纯度相对较高。用 50mM 的 HSO 从 100% HPAA 预处理的玉米芯中获得了最高的葡萄糖产率（79.1%）。最后，从 1kg 的玉米芯中获得了 58.3g 的 XOS、186.9g 的葡萄糖和 56.4g 的木糖。本研究提供了一种有前途的方法，可在不分离木聚糖的情况下从玉米芯中生产 XOS 和单糖。
