新型含硒壳聚糖衍生物的合成、表征及生物学评价。
Synthesis, characterization, and biological evaluation of novel selenium-containing chitosan derivatives.
机构信息
School of Food Science and Technology, State Key Laboratory of Food Science and Technology, Jiangnan University, Jiangsu, China; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Jiangsu, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, China.
School of Food Science and Technology, State Key Laboratory of Food Science and Technology, Jiangnan University, Jiangsu, China; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.
出版信息
Carbohydr Polym. 2022 May 15;284:119185. doi: 10.1016/j.carbpol.2022.119185. Epub 2022 Jan 29.
Though great efforts have been made to develop selenium polysaccharides with unique properties using HNO-NaSeO methods, the Se content is still low due to the poor esterification efficiency of HSeO. Herein, selenodiacetic acid (SA) was introduced into chitosan (CS) to synthesize O-selenodiacetyl chitosan (OSAC) and chitosan-ammonium selenodiacetate (CASA) by covalent and non-covalent interaction, respectively. The obtained CS derivatives were characterized by UV-vis, FTIR, H NMR, XPS, TGA, and XRD spectra, and the OSAC and CASA showed high Se content up to 15,720 ± 475 and 26,363 ± 698 μg/g. The OSAC and CASA demonstrated increased antioxidant activities compared to the CS in DPPH and ABTS free radical scavenging assays. Moreover, they exhibited a potent anticancer effect on HepG2 cells with the IC values of 0.918 and 1.459 μg/mL. Taken together, this study provides a promising strategy for the design of novel selenium polysaccharides with high Se content and greater biological activity.
尽管已经付出了巨大的努力，采用 HNO-NaSeO 方法开发具有独特性质的硒多糖，但由于 HSeO 的酯化效率差，硒含量仍然较低。在此，将乙二酸（SA）引入壳聚糖（CS）中，通过共价和非共价相互作用分别合成 O-硒代乙二酰壳聚糖（OSAC）和壳聚糖-铵硒代乙二酸盐（CASA）。通过 UV-vis、FTIR、H NMR、XPS、TGA 和 XRD 光谱对所得 CS 衍生物进行了表征，OSAC 和 CASA 的硒含量高达 15720±475μg/g 和 26363±698μg/g。与 CS 相比，OSAC 和 CASA 在 DPPH 和 ABTS 自由基清除测定中显示出更高的抗氧化活性。此外，它们对 HepG2 细胞表现出强大的抗癌作用，IC 值分别为 0.918 和 1.459μg/mL。总之，该研究为设计具有高硒含量和更大生物活性的新型硒多糖提供了一种有前途的策略。
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