Department of Dentistry-Oral Health Institute, Hamad Medical Corporation, Doha, Qatar; College of Dental Medicine, Qatar University, Doha, Qatar.
Faculty of Engineering and Natural Sciences, International University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Photodiagnosis Photodyn Ther. 2022 Jun;38:102804. doi: 10.1016/j.pdpdt.2022.102804. Epub 2022 Mar 11.
The aim of this in vitro study was to assess the efficacy of antimicrobial photodynamic therapy (aPDT), neodymium-doped yttrium aluminium garnet (Nd:YAG) laser, and two other disinfecting agents against P. gingivalis and T. forsythia that colonized over zirconia ceramics and to evaluate if the disinfecting protocols result in zirconia surface alteration.
The experiment was conducted on pre-sintered, commercially available Yttria Stabilized Zirconia (Y-TZP) ceramic blocks. The bacterial strains of P. gingivalis and T. forsythia were used to contaminate the zirconia specimens. The infected zirconia specimens were randomly divided into 4 groups (n=20/group): aPDT, Nd:YAG laser, hydrogen peroxide (HO), and chlorhexidine groups. The viability of bacteria was assayed using the MTT protocol. The surface roughness (R) of all zirconia specimens was estimated using a profilometer and a drop-shape analyzer was used to evaluate the contact angle using a special sessile drop method.
A statistically significant reduction by the aPDT group is noticed for P. gingivalis and T. forsythia species compared to other disinfection methods (p<0.05). The second highest reduction was seen for chlorhexidine followed by Nd:YAG laser. The least reduction was demonstrated for the hydrogen peroxide group. Statistically, there was no significant difference in R scores between the four groups. However, the contact angles were significantly reduced from the zirconia specimens after the aPDT method which indicates a hydrophilicity increase compared to other groups. The SFE scores of all decontamination protocols from highest to lowest were aPDT (41.68), chlorhexidine (39.83), Nd:YAG (34.52), and H2O2 (29.88).
Antimicrobial photodynamic therapy demonstrated a high antibacterial efficacy over zirconia ceramic surface without altering surface topography.
本体外研究旨在评估抗菌光动力疗法（aPDT）、掺钕钇铝石榴石（Nd:YAG）激光以及另外两种消毒剂对定植于氧化锆陶瓷表面的牙龈卟啉单胞菌和福赛拟杆菌的疗效，并评估消毒方案是否会导致氧化锆表面发生变化。
该实验在预烧结的商用氧化钇稳定氧化锆（Y-TZP）陶瓷块上进行。牙龈卟啉单胞菌和福赛拟杆菌的菌株用于污染氧化锆标本。将感染的氧化锆标本随机分为 4 组（n=20/组）：aPDT 组、Nd:YAG 激光组、过氧化氢（HO）组和洗必泰组。使用 MTT 方案检测细菌活力。使用轮廓仪评估所有氧化锆标本的表面粗糙度（R），并使用滴形分析仪通过特殊的固着液滴法评估接触角。
与其他消毒方法相比，aPDT 组对牙龈卟啉单胞菌和福赛拟杆菌的减少具有统计学意义（p<0.05）。洗必泰组次之，Nd:YAG 激光组次之。过氧化氢组的减少最少。四组之间 R 评分无统计学差异。然而，与其他组相比，aPDT 法后氧化锆标本的接触角显著降低，表明亲水性增加。所有消毒方案的 SFE 评分从高到低依次为 aPDT（41.68）、洗必泰（39.83）、Nd:YAG（34.52）和 H2O2（29.88）。
抗菌光动力疗法对氧化锆陶瓷表面表现出高效的抗菌作用，而不会改变表面形貌。
