Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Guangzhou Hanyun Pharmaceutical Technology Co. Ltd, Guangzhou, China.
Food Funct. 2022 Apr 4;13(7):3917-3930. doi: 10.1039/d1fo02832d.
The β-glucan from is a potent adjuvant that exhibits a broad spectrum of biological activities and health benefits, and different processes have been established to prepare active β-glucan from yeast. However, studies concerning the effect of β-1,6-glucanase enzymolysis on the structure and immunomodulatory activity of yeast β-1,3-glucan are scarce. In this study, we aim to develop a novel enzymatic process for the preparation of immunologically active β-glucan (BYG) from baker's yeast using a β-1,6-glucanase GluM. The β-1,6-glucan in fungal cell wall was specifically hydrolyzed by GluM, and resulted in cell wall decomposition and β-glucan release. Batch production of BYG was realized with 17.8% yield, 85.3% purity and 75.4% recovery rate. Structural characterization indicated that BYG exhibits rod-like structures with natural triplex and nanoparticle-like substructures compared with the commercial Glucan 300. BYG ameliorated inflammation in a DSS-induced mouse model of colitis through inhibiting oxidative stress (NO, MDA and MPO), inflammatory mediators (NLRP3, ASC, caspase-1, iNOS and COX-2), and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-γ), increasing the expression levels of tight junction proteins (ZO-1, occludin and claudin-1) and modulating the production of gut microbiota-synthesized SCFAs compared to the control. Our results showed that yeast β-1,3-glucan prepared with β-1,6-glucanase exhibits structural integrity that is responsible for its favorable immunomodulatory activity.
从 中提取的β-葡聚糖是一种有效的佐剂，具有广泛的生物活性和健康益处，已经建立了不同的工艺来从酵母中制备活性β-葡聚糖。然而，关于β-1,6-葡聚糖酶酶解对酵母β-1,3-葡聚糖结构和免疫调节活性的影响的研究还很少。在这项研究中，我们旨在开发一种使用β-1,6-葡聚糖酶 GluM 从面包酵母中制备免疫活性β-葡聚糖（BYG）的新型酶解工艺。真菌细胞壁中的β-1,6-葡聚糖被 GluM 特异性水解，导致细胞壁分解和β-葡聚糖释放。通过 17.8%的产率、85.3%的纯度和 75.4%的回收率实现了 BYG 的批生产。结构表征表明，与商业 Glucan 300 相比，BYG 具有天然三聚体和纳米颗粒状亚结构的棒状结构。BYG 通过抑制氧化应激（NO、MDA 和 MPO）、炎症介质（NLRP3、ASC、caspase-1、iNOS 和 COX-2）和促炎细胞因子（IL-1β、IL-6、TNF-α、IFN-γ），增加紧密连接蛋白（ZO-1、occludin 和 claudin-1）的表达水平，并调节肠道微生物群合成的 SCFAs 的产生，从而改善 DSS 诱导的结肠炎小鼠模型中的炎症。我们的结果表明，用β-1,6-葡聚糖酶制备的酵母β-1,3-葡聚糖具有结构完整性，这是其良好的免疫调节活性的原因。
