Javmen Artur, Nemeikaitė-Čėnienė Aušra, Bratchikov Maksim, Grigiškis Saulius, Grigas Fortūnatas, Jonauskienė Irena, Zabulytė Danguolė, Mauricas Mykolas
Department of Immunology, State Scientific Research Institute Centre for Innovative Medicine, Vilnius, Lithuania JSC Biocentras, Vilnius, Lithuania
Department of Immunology, State Scientific Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.
In Vivo. 2015 May-Jun;29(3):359-63.
β-Glucan is one of the most abundant polymers in nature and has been established as an immunomodulator. This compound has notable physiological effects on mammalian immune systems, including anti-tumor and anti-infective activities and can activate the immune response. It is considered that the immune-stimulating activities of β-glucan can depend on physicochemical parameters, such as molecular size. Saccharomyces cerevisiae, also known as baker's yeast, is a frequently used source of β-glucan. The aim of the experiments was to investigate how different Saccharomyces cerevisiae β-glucan preparations with different molecular size affect interferon-gamma (IFN-γ) production in BALB/c mice.
In vivo and in vitro BALB/c mouse models were used for the investigations. Different β-glucan preparations were orally administrated in the in vivo experiments. IFN-γ production in BALB/c mice was analyzed by enzyme-linked immunosorbent assay and measuring interferon-γ RNA concentration.
The results showed that orally-administered β-glucan from S. cerevisiae enhanced IFN-γ production in BALB/c mice in the in vivo model, but not by mouse leukocytes in vitro. Moreover, water-soluble β-glucan enhanced IFN-γ production more effectively than did particulate β-glucan.
IFN-γ plays an important role in immunity against viral and bacterial infections. Our experiments have shown that β-glucan preparations enhance IFN-γ production in BALB/c mice and can be potentially used for immune system stimulation in mammals. Current results may be used to develop soluble β-glucan nutritional supplements.
β-葡聚糖是自然界中含量最丰富的聚合物之一，已被确认为一种免疫调节剂。该化合物对哺乳动物免疫系统具有显著的生理作用，包括抗肿瘤和抗感染活性，并且能够激活免疫反应。据认为，β-葡聚糖的免疫刺激活性可能取决于物理化学参数，如分子大小。酿酒酵母，也被称为面包酵母，是β-葡聚糖常用的来源。本实验的目的是研究不同分子大小的酿酒酵母β-葡聚糖制剂如何影响BALB/c小鼠中γ-干扰素（IFN-γ）的产生。
体内和体外BALB/c小鼠模型用于本研究。在体内实验中口服给予不同的β-葡聚糖制剂。通过酶联免疫吸附测定法和测量γ-干扰素RNA浓度来分析BALB/c小鼠中IFN-γ的产生。
结果表明，在体内模型中，口服酿酒酵母来源的β-葡聚糖可增强BALB/c小鼠中IFN-γ的产生，但在体外对小鼠白细胞无此作用。此外，水溶性β-葡聚糖比颗粒状β-葡聚糖更有效地增强了IFN-γ的产生。
IFN-γ在抵抗病毒和细菌感染的免疫中起重要作用。我们的实验表明，β-葡聚糖制剂可增强BALB/c小鼠中IFN-γ的产生，并可能潜在地用于刺激哺乳动物的免疫系统。目前的结果可用于开发可溶性β-葡聚糖营养补充剂。
