Department of Oral Biology, Oral Science Research Center, Department of Applied Life Science, The Graduate School, BK21 Four Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
Department of Oral Biochemistry, School of Dentistry, Jeonbuk National University, Jeonju, Republic of Korea.
Gut Microbes. 2022 Jan-Dec;14(1):2044721. doi: 10.1080/19490976.2022.2044721.
is the major risk factor for gastric cancer. harboring the type IV secretion system (T4SS) and its effector CagA encoded on the pathogenicity Island (PAI) increases the risk. PMSS1 has a multi- genotype, modulating copy number dynamically from zero to four copies. To examine the effect of the immune response on copy number change, we utilized a mouse model with different immune status. PMSS1 recovered from mice, lacking functional T or B cells, retained more copies. PMSS1 recovered from mice, showing intense inflammation, had fewer copies compared to those recovered from wild-type mice. Moreover, copy number of PMSS1 recovered from wild-type and mice was positively correlated with the capacity to induce IL-8 secretion at four weeks of infection. Since recombination in influences T4SS function, including CagA translocation and IL-8 induction, we constructed a multiple linear regression model to predict -induced IL-8 expression based on copy number and recombination status; induces more IL-8 secretion when the strain has more copies and intact . This study shows that PMSS1 in mice with less intense immune response possess higher copy number than those infected in mice with more intense immune response and thus the multi- genotype, along with recombination, functions as an immune-sensitive regulator of virulence.
幽门螺杆菌是胃癌的主要危险因素。携带第四型分泌系统（T4SS）及其效应因子 CagA 的致病岛（PAI）增加了风险。PMSS1 具有多种基因型，可动态调节从零到四个拷贝的拷贝数。为了研究免疫反应对拷贝数变化的影响，我们利用具有不同免疫状态的小鼠模型进行了研究。从缺乏功能性 T 或 B 细胞的小鼠中回收的 PMSS1 保留了更多的拷贝数。与从野生型小鼠中回收的 PMSS1 相比，从具有强烈炎症的小鼠中回收的 PMSS1 的拷贝数较少。此外，从野生型和小鼠中回收的 PMSS1 的拷贝数与感染 4 周时诱导 IL-8 分泌的能力呈正相关。由于在影响 T4SS 功能，包括 CagA 易位和 IL-8 诱导，我们构建了一个多元线性回归模型，根据拷贝数和重组状态预测 - 诱导的 IL-8 表达；当菌株具有更多的拷贝数和完整的时，会诱导更多的 IL-8 分泌。这项研究表明，在免疫反应较弱的小鼠中，PMSS1 比在免疫反应较强的小鼠中感染的 PMSS1 具有更高的拷贝数，因此多基因型与重组一起，作为一种对毒力具有免疫敏感性的调节因子。
