Department of Dermatology and Allergy, Herlev and Gentofte Hospital, University of Copenhagen, Hellerup, DK, 2900, Denmark.
Copenhagen Research Group for Inflammatory Skin (CORGIS), Hellerup, Denmark.
Br J Dermatol. 2022 Jul;187(1):89-98. doi: 10.1111/bjd.21245. Epub 2022 May 3.
Accumulating evidence supports the findings of an altered gut microbiota in patients with autoimmune disease. However, existing studies on the role of the gut microbiota in patients with psoriasis have demonstrated conflicting results and have mainly been based on 16s rRNA gene sequencing analysis.
To examine whether the gut microbiota of patients with psoriasis was altered in composition and functional potentials compared with healthy controls, and as a second approach compared with healthy cohabitant partners. A further aim was to investigate relationships to disease severity, and seasonal impact on the gut microbiota.
In a case-control study, 126 faecal samples were collected from a sample of 53 systemically untreated patients with plaque psoriasis; 52 healthy controls matched for age, sex and body mass index; and 21 cohabitant partners. A subpopulation of 18 patients with psoriasis and 19 healthy controls continued in a longitudinal study, where four to six faecal samples were collected over 9-12 months. The gut microbiota was characterized using shotgun metagenomic sequencing analysis.
A significantly lower richness (P = 0·007) and difference in community composition (P = 0·01) of metagenomic species was seen in patients with psoriasis compared with healthy controls, and patients with psoriasis had a lower microbial diversity than their partners (P = 0·04). Additionally, the functional richness was decreased in patients with psoriasis compared with healthy controls (P = 0·01) and partners (P = 0·05). Increased disease severity was correlated with alterations in taxonomy and function, with a slight tendency towards a lower richness of metagenomic species, albeit not significant (P = 0·08). The seasonal analysis showed no shifts in community composition in healthy controls or in patients with psoriasis.
The findings of a different gut microbiota in composition and functional potentials between patients with psoriasis and healthy controls support a linkage between the gut microbiota and psoriasis. These findings need to be validated in larger studies, and a potential causal relationship between the gut microbiota and psoriasis still needs to be shown.
越来越多的证据支持自身免疫性疾病患者的肠道微生物群发生改变的观点。然而，现有的关于银屑病患者肠道微生物群作用的研究结果相互矛盾，并且主要基于 16s rRNA 基因测序分析。
检测银屑病患者的肠道微生物群在组成和功能潜能上是否与健康对照组不同，并与健康同居伴侣进行比较。进一步的目的是研究与疾病严重程度的关系，以及肠道微生物群对季节性的影响。
在一项病例对照研究中，我们从 53 例未经系统治疗的斑块状银屑病患者中采集了 126 份粪便样本；52 名年龄、性别和体重指数相匹配的健康对照组；21 名同居伴侣。18 例银屑病患者和 19 名健康对照组的一个亚组继续进行纵向研究，在 9-12 个月的时间内采集了 4 到 6 份粪便样本。使用 shotgun 宏基因组测序分析来描述肠道微生物群。
与健康对照组相比，银屑病患者的微生物物种丰富度显著降低（P = 0·007），群落组成也存在差异（P = 0·01），且银屑病患者的微生物多样性低于其伴侣（P = 0·04）。此外，与健康对照组（P = 0·01）和伴侣（P = 0·05）相比，银屑病患者的功能丰富度也降低了。疾病严重程度的增加与分类和功能的改变相关，尽管不显著（P = 0·08），但微生物物种的丰富度略有降低。季节性分析显示，健康对照组或银屑病患者的群落组成没有变化。
银屑病患者肠道微生物群在组成和功能潜能上与健康对照组存在差异的发现，支持了肠道微生物群与银屑病之间存在联系。这些发现需要在更大的研究中得到验证，并且需要证明肠道微生物群与银屑病之间存在潜在的因果关系。
