Caira Simonetta, Pinto Gabriella, Picariello Gianluca, Vitaglione Paola, De Pascale Sabrina, Scaloni Andrea, Addeo Francesco
Proteomics, Metabolomics & Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment (ISPAAM), National Research Council, Piazzale Enrico Fermi 1, 80055 Portici (NA), Italy.
Department of Chemical Sciences, University of Naples "Federico II", 80126 Naples, Italy.
Food Chem. 2022 Aug 15;385:132663. doi: 10.1016/j.foodchem.2022.132663. Epub 2022 Mar 12.
A dedicated two-step purification procedure prior to nanoliquid chromatography-electrospray-tandem mass spectrometry analysis enabled the identification of bovine milk-derived peptides absorbed and circulating in the plasma of three healthy volunteers who received 250 mL of pasteurized milk after a 10-days washout. The appearance and clearance of milk peptides in plasma were monitored at various time points. Overall, 758, 273 and 212 unique peptides derived from 15, 15 and 18 bovine milk proteins, respectively, were identified in the plasma of these volunteers, evidencing a substantial inter-individual variability. Peptides encrypting possible bioactive and/or immunogenic molecules originating from caseins, β-lactoglobulin and minor milk proteins were detected. Peptide representation data revealed the combined action of endoproteases involved in primary hydrolysis during gastroduodenal digestion and exopeptidases that hydrolyse peptides in the small intestine. It remains to be established whether the half-life and concentration ranges of circulating milk-derived peptides may have any impacts on human health.
在进行纳升液相色谱-电喷雾串联质谱分析之前，采用专门的两步纯化程序，得以鉴定出三名健康志愿者血浆中吸收并循环的源自牛乳的肽段。这三名志愿者在经过10天的洗脱期后，饮用了250毫升巴氏杀菌乳。在不同时间点监测血浆中乳肽的出现和清除情况。总体而言，在这些志愿者的血浆中分别鉴定出了源自15种、15种和18种牛乳蛋白的758种、273种和212种独特肽段，这表明个体间存在很大差异。检测到了源自酪蛋白、β-乳球蛋白和少量乳蛋白的可能具有生物活性和/或免疫原性的分子的加密肽段。肽段代表性数据揭示了胃十二指肠消化过程中参与初级水解的内切蛋白酶和在小肠中水解肽段的外切蛋白酶的联合作用。循环乳源肽段的半衰期和浓度范围是否会对人体健康产生任何影响，仍有待确定。
