Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University (Shandong Academy of Medical Sciences), Tai'an, China.
School of Basic Medical Sciences, Shandong First Medical University (Shandong Academy of Medical Sciences), Tai'an, China.
Parasit Vectors. 2022 Mar 15;15(1):89. doi: 10.1186/s13071-022-05214-w.
Ticks are important vectors and transmit diverse pathogens, including protozoa, viruses, and bacteria. Tick-borne diseases can cause damage to both human health and the livestock industries. The control and prevention of ticks and tick-borne diseases has relied heavily on acaricides.
In the present study, using a high-throughput RNA sequencing (RNA-Seq) technique, we performed a comprehensive time-series transcriptomic analysis throughout the embryogenesis period of Rhipicephalus turanicus.
Altogether, 127,157 unigenes were assembled and clustered. Gene expression differences among the embryonic stages demonstrated that the most differentially expressed genes (DEGs) were observed in the comparisons of early embryonic stages (RTE5 vs. RTE10, 9726 genes), and there were far fewer DEGs in later stages (RTE25 vs. RTE30, 2751 genes). Furthermore, 16 distinct gene modules were identified according to weighted gene co-expression network analysis (WGCNA), and genes in different modules displayed stage-specific characteristics. Gene Ontology (GO) annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment suggested that some genes involved in organ and tissue formation were significantly upregulated in the early embryonic developmental stages, whereas metabolism-related pathways were more enriched in the later embryonic developmental stages.
These transcriptome studies revealed gene expression profiles at different stages of embryonic development, which would be useful for interrupting the embryonic development of ticks and disrupting the transmission of tick-borne diseases.
蜱是重要的载体，可传播多种病原体，包括原生动物、病毒和细菌。蜱传疾病会对人类健康和畜牧业造成损害。蜱和蜱传疾病的控制和预防主要依赖杀蜱剂。
本研究采用高通量 RNA 测序（RNA-Seq）技术，对草原革蜱胚胎发生期进行了全面的时间序列转录组分析。
共组装和聚类了 127157 个基因。胚胎阶段的基因表达差异表明，早期胚胎阶段（RTE5 与 RTE10，9726 个基因）的差异表达基因（DEGs）最多，而后期阶段（RTE25 与 RTE30，2751 个基因）的 DEGs 则较少。此外，根据加权基因共表达网络分析（WGCNA），鉴定出 16 个不同的基因模块，不同模块中的基因表现出特定阶段的特征。基因本体（GO）注释和京都基因与基因组百科全书（KEGG）通路富集表明，一些参与器官和组织形成的基因在早期胚胎发育阶段显著上调，而代谢相关通路在后期胚胎发育阶段更为丰富。
这些转录组研究揭示了胚胎发育不同阶段的基因表达谱，这对于阻断蜱的胚胎发育和中断蜱传疾病的传播将非常有用。
