School of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.
School of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.
Bioresour Technol. 2022 May;351:126998. doi: 10.1016/j.biortech.2022.126998. Epub 2022 Mar 12.
The effect of 50 mg/L nitrogen-doped graphene (N-G) on anammox microbial guild was studied by metagenomics in this paper. The continuous experiment results showed the average NRE improved by 17.57% with N-G addition. The metagenomic analysis revealed N-G significantly increased the relative abundance of dominant AnAOB (Candidatus Kuenenia) from 18.10% to 28.30%. And the FISH assay further manifested N-G promoted the growth of AnAOB biomass. Meanwhile, metagenomics indicated that N-G enriched the abundance of genes (Hzs, Hdh, NosZ, NorB, NirK, NirS and NrfA) involved in nitrogen metabolism to varying degrees. Furthermore, N-G not only improved the microbial functionality in terms of "Metabolism", but markedly upregulated the abundance of c-di-GMP synthesized genes and genes related to quinolone signal molecule, which contributed to more EPS content and better sludge settleability. In brief, this study provided a novel perspective for anammox biomass enrichment, which may be valuable for practical engineering applications of anammox.
本文通过宏基因组学研究了 50mg/L 氮掺杂石墨烯（N-G）对厌氧氨氧化菌群落的影响。连续实验结果表明，添加 N-G 可使平均 NRE 提高 17.57%。宏基因组分析显示，N-G 可使优势厌氧氨氧化菌（Candidatus Kuenenia）的相对丰度从 18.10%显著增加到 28.30%。FISH 检测进一步表明，N-G 促进了厌氧氨氧化菌生物量的生长。同时，宏基因组学表明，N-G 可不同程度地富集与氮代谢相关的基因（Hzs、Hdh、NosZ、NorB、NirK、NirS 和 NrfA）的丰度。此外，N-G 不仅提高了“代谢”方面的微生物功能，而且显著上调了 c-di-GMP 合成基因和与喹诺酮信号分子相关的基因的丰度，这有助于提高 EPS 含量和改善污泥沉降性能。总之，本研究为厌氧氨氧化菌的生物量富集提供了新的视角，可能对厌氧氨氧化的实际工程应用具有重要价值。
