College of Resources & Civil Engineering, Northeastern University, Shenyang 110819, PR China.
College of Resources & Civil Engineering, Northeastern University, Shenyang 110819, PR China.
Bioresour Technol. 2023 Jul;380:129116. doi: 10.1016/j.biortech.2023.129116. Epub 2023 May 1.
This paper aims to investigate the function of a pulsed electric field (PEF) in the anaerobic ammonia oxidation (anammox) process after adding certain chemical oxygen demand (COD) through integrated network and metagenomics analyses. The findings showed that the presence of COD was detrimental to anammox, but PEF could significantly reduce the adverse effect. The total nitrogen removal in the reactor for applying PEF was 16.99% higher on average than the reactor for only dosing COD. Additionally, PEF upgraded the abundance of anammox bacteria subordinate to the phylum Planctomycetes by 9.64%. The analysis of molecular ecological networks promulgated that PEF resulted in an increase in network scale and topology complexity, thereby boosting the potential collaboration of the communities. Metagenomics analyses demonstrated that PEF dramatically promoted anammox central metabolism in the presence of COD, specifically enhancing pivotal N functional genes (hzs, hdh, amo, hao, nas, nor and nos).
本文旨在通过整合网络和宏基因组学分析，研究在添加一定化学需氧量 (COD) 后，脉冲电场 (PEF) 在厌氧氨氧化 (anammox) 工艺中的作用。研究结果表明，COD 的存在对 anammox 不利，但 PEF 可显著降低其不利影响。在施加 PEF 的反应器中，总氮去除率平均比仅投加 COD 的反应器高 16.99%。此外，PEF 将从属盘菌门的 anammox 细菌丰度提高了 9.64%。分子生态网络分析表明，PEF 导致网络规模和拓扑复杂性增加，从而提高了群落的潜在协同作用。宏基因组学分析表明，PEF 在 COD 存在的情况下显著促进了 anammox 的中心代谢，特别是增强了关键 N 功能基因 (hzs、hdh、amo、hao、nas、nor 和 nos)。
