Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Miguel Othón de Mendizábal 699, C.P. 07360, Ciudad de México, México.
Laboratory of Cellular Reprogramming and Chronic-Degenerative Diseases, Department of Physiology, School of Medicine, UNAM, Mexico City, Mexico.
Plant Foods Hum Nutr. 2022 Mar;77(1):37-43. doi: 10.1007/s11130-022-00957-4. Epub 2022 Mar 15.
The anti-inflammatory effects of Aloe vera (AV), polysaccharide extract from AV, and extracts from the digestion and colonic fermentation of AV were evaluated using an immortal astrocyte cell line (U373 MG) that develops a neuro-inflammatory profile. Cell viability and inflammatory markers were assessed after stimulation with neuropeptide substance P (SP) that activates the pro-inflammatory MAPK (mitogen-activated protein kinase) pathway. Cell viability after SP treatment was over 50% at 10 mg/mL AV, polysaccharide extract from AV, extracts from the digestion: non-digestible fraction of AV non-digestible fraction of polysaccharide extract from AV and extracts from the colonic fermentation of AV, at 4 and 24 h. Moreover, cells exposed to SP and treated with these extracts showed lower protein-activated ERK1/ERK2 (extracellular signal-regulated kinases 1 and 2), p38 (MAPK protein p38), and NFκB (nuclear factor κB) levels with respect to the SP-stimulated control. Inflammation inhibition by extracts of polysaccharide extract from AV and extracts from the colonic fermentation of AV, at 24 h in the study of p38 was not as statistically significant in ERK1/ERK2 and NFκB. Nevertheless, there was a significant decrease (p < 0.05) in pro-inflammatory cytokine IL-6 levels in cells exposed to all samples. Samples with extracts from the colonic fermentation of AV, at 4 or 24 h showed the highest inhibitory effect on IL-6 production.
采用永生化神经胶质瘤细胞系（U373MG）评估了芦荟（AV）、AV 多糖提取物、AV 消化和结肠发酵提取物的抗炎作用，该细胞系会产生神经炎症特征。用神经肽物质 P（SP）刺激后，评估细胞活力和炎症标志物，SP 可激活促炎丝裂原活化蛋白激酶（MAPK）途径。在 10mg/mL AV、AV 多糖提取物、消化提取物（AV 不可消化部分）、非消化多糖提取物部分和 AV 结肠发酵提取物中，SP 处理后细胞活力在 4 和 24 小时时均超过 50%。此外，与 SP 刺激的对照组相比，暴露于 SP 并经这些提取物处理的细胞中，蛋白激活的细胞外信号调节激酶 1/2（ERK1/ERK2）、p38（MAPK 蛋白 p38）和核因子 κB（NFκB）水平较低。在研究 p38 时，24 小时时 AV 多糖提取物和 AV 结肠发酵提取物的提取物对炎症的抑制作用在 ERK1/ERK2 和 NFκB 方面并不具有统计学意义。然而，所有样品中暴露的细胞中促炎细胞因子 IL-6 水平均显著降低（p<0.05）。在 4 或 24 小时时，具有 AV 结肠发酵提取物的样品对 IL-6 产生的抑制作用最强。
