GC-MS 分析与曼陀罗对茄丝核菌的抑菌活性
GC-MS analysis & antifungal activity of Datura metel L. against Rhizoctonia solani Kuhn.
机构信息
Department of Botany, Lahore College for Women University, 54000 Lahore, Pakistan.
Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
出版信息
An Acad Bras Cienc. 2022 Mar 11;94(1):e20200851. doi: 10.1590/0001-3765202220200851. eCollection 2022.
The current study was designed to evaluate the antifungal properties of Datura metel L. against Rizoctonia solani Kuhn. To achieve this objective, six concentrations of leaves & stem methanol extract of D. metel viz. 1%, 1.5%, 2%, 2.5%, 3% & 3.5% were tested against R. solani in vitro. Leaf extract of D. metel was found more effective as its 3.5% concentration caused 75% retardation in test fungal growth as compared to the stem extract. D. metel methanolic leaf extract was fractioned between n-butanol, n-hexane, chloroform & ethyl acetate & bioactivities of isolated fractions were tested against R. solani. The chloroform fraction was found highly effective, as its concentrations 0.1% & 0.01% caused 27% & 21% growth inhibition respectively. So, this particular chloroform fraction was further analyzed to identify various chemical constituents through GC-MS (Gas chromatography mass spectroscopic) analysis. Twelve phyto-constituents viz. eugenol, 2-pentadecanone 6,10,14 trimethyl, pentadecanoic acid, pentadecanoic acid, 1 4-methyl- methyl ester, phytol, 9,12,15-octadecatrienoic acid, heptacosane, n-hexadecanoic, 6-octadecanoic acid, 9, 12 octadecanoic acid, dodecanoic & tetradecanoic acids were identified. So, the present study concluded that the presence of these bioactive constituents make D. metel as an effective antifungal agent against R. solani.
本研究旨在评估曼陀罗甲醇提取物对核盘菌的抗真菌特性。为了达到这个目的，我们测试了曼陀罗的叶和茎甲醇提取物的六个浓度，即 1%、1.5%、2%、2.5%、3%和 3.5%，对抗核盘菌。曼陀罗叶提取物的效果更为显著，因为其 3.5%浓度可使测试真菌生长的延缓率达到 75%，而茎提取物的延缓率为 55%。曼陀罗的甲醇叶提取物被分离为正丁醇、正己烷、氯仿和乙酸乙酯，并测试了分离得到的各馏分对核盘菌的生物活性。氯仿馏分的效果最为显著，其浓度为 0.1%和 0.01%时，分别可导致 27%和 21%的生长抑制。因此，这个特殊的氯仿馏分进一步通过 GC-MS（气相色谱-质谱联用）分析来鉴定各种化学成分。通过 GC-MS 分析鉴定出了 12 种植物成分，分别为丁香酚、2-十五烷酮 6、10、14 三甲基、十五烷酸、十五烷酸、1 4-甲基-甲酯、叶绿醇、9、12、15-十八碳三烯酸、十七烷、正十六烷酸、6-十八烷酸、9、12 十八烷酸、十二烷酸和十四烷酸。因此，本研究得出结论，这些生物活性成分的存在使曼陀罗成为一种有效的抗核盘菌的真菌剂。
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