Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Deanship of Scientific Research, Umm Al-Qura University, Makkah, Saudi Arabia.
Microsc Res Tech. 2022 Jul;85(7):2467-2485. doi: 10.1002/jemt.24102. Epub 2022 Mar 16.
Microscopic pollen characters from 19 species of family Asteraceae collected from different areas of the Potohar Plateau of Pakistan have been investigated using light and scanning electron microscopy. This study uses microscopic techniques to provide detailed information on pollen and its exine structure based on qualitative and quantitative characters. The present study has observed three types of pollen grains based on their apertures, that is, trizonocolporate, tetrazonocolporate, and tricolporate. Exine sculpturing is echinate in all studied taxa. Some species also exhibit Lacunae which differ in their shape and sizes. The size of the spines and the number of spines between the colpi play a vital role in differentiating the species of the same genus. Pollen fertility ranges from 94.3 to 74.19, showing that these species are well established in the study area. For the correct identification of species, a light and scanning electron microscopy was used that provides sufficient information for a taxonomist to distinguish species taxonomically. RESEARCH HIGHLIGHTS: Light and Scanning electron microscope is comparatively used to investigate the pollen characters of some species of the family Asteraceae. A scanning electron microscopy is used to study the micro-morphological characters of pollen grains. Qualitative and quantitative characters of pollen were studied.
利用光学和扫描电子显微镜研究了来自巴基斯坦波特瓦尔高原不同地区的 19 种菊科植物的微观花粉特征。本研究使用微观技术，基于定性和定量特征，提供有关花粉及其外壁结构的详细信息。本研究根据孔的类型观察到三种花粉粒，即三沟花粉、四射花粉和三沟花粉。所有研究的分类群的外壁纹饰均为粒状。一些物种还具有不同形状和大小的陷孔。脊的大小和沟间脊的数量在区分同一属的物种方面起着至关重要的作用。花粉活力范围从 94.3 到 74.19，表明这些物种在研究区域中已很好地建立。为了正确识别物种，使用了光学和扫描电子显微镜，这为分类学家提供了足够的信息，以便在分类学上区分物种。研究亮点： 比较使用光学和扫描电子显微镜研究了菊科某些物种的花粉特征。 扫描电子显微镜用于研究花粉粒的微观形态特征。 研究了花粉的定性和定量特征。
