沙门氏菌肠毒素的制备及部分纯化
Production and partial purification of Salmonella enterotoxin.
作者信息
Sedlock D M, Koupal L R, Deibel R H
出版信息
Infect Immun. 1978 May;20(2):375-80. doi: 10.1128/iai.20.2.375-380.1978.
Abstract
By using a strain of Salmonella typhimurium, we detected the presence of an enterotoxin, as determined by the rabbit ileal loop assay, in various complex and defined media. The enterotoxin was concentrated by ultrafiltration of culture supernatant fluids and eluted in and adjacent to the void volume of a Sephadex G-100 column. This suggested that the enterotoxic factor was of a relatively high molecular weight, and additional evidence indicated it was heterogeneous in size. Further chromatography, using a diethylaminoethyl-cellulose anion exchanger, facilitated at least a 50-fold purification of the Salmonella enterotoxin.
摘要
通过使用鼠伤寒沙门氏菌菌株，我们在各种复杂和特定培养基中检测到了一种肠毒素的存在，这是通过兔回肠袢试验确定的。通过对培养上清液进行超滤浓缩肠毒素，并在葡聚糖G - 100柱的空体积及附近洗脱。这表明肠毒素因子分子量相对较高，另外的证据表明其大小不均一。使用二乙氨基乙基纤维素阴离子交换剂进行进一步色谱分析，使得沙门氏菌肠毒素的纯化至少提高了50倍。
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