线粒体膜电位检测。
Mitochondrial Membrane Potential Assay.
机构信息
National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA.
Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, Washington, DC, USA.
出版信息
Methods Mol Biol. 2022;2474:11-19. doi: 10.1007/978-1-0716-2213-1_2.
Abstract
Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in various types of cells, such as HepG2, HepaRG, and AC16 cells. A homogenous cell-based MMP assay has been optimized and performed in a 1536-well plate format, which can be used to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP.
摘要
线粒体功能是细胞健康的一个关键指标，可以通过监测线粒体膜电位（MMP）的变化来评估。阳离子荧光染料是常用的评估 MMP 的工具。我们使用一种水溶性的线粒体膜电位指示剂（m-MPI）来检测各种类型的细胞（如 HepG2、HepaRG 和 AC16 细胞）中 MMP 的变化。我们已经优化了基于细胞的均相 MMP 测定法，并在 1536 孔板格式中进行了测试，该方法可用于通过评估化合物对 MMP 的影响来筛选几种化合物库的线粒体毒性。
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