NIH Chemical Genomics Center, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA.
Drug Discov Today. 2013 Aug;18(15-16):716-23. doi: 10.1016/j.drudis.2013.05.015. Epub 2013 May 31.
Since its establishment in 2008, the US Tox21 inter-agency collaboration has made great progress in developing and evaluating cellular models for the evaluation of environmental chemicals as a proof of principle. Currently, the program has entered its production phase (Tox21 Phase II) focusing initially on the areas of modulation of nuclear receptors and stress response pathways. During Tox21 Phase II, the set of chemicals to be tested has been expanded to nearly 10,000 (10K) compounds and a fully automated screening platform has been implemented. The Tox21 robotic system combined with informatics efforts is capable of screening and profiling the collection of 10K environmental chemicals in triplicate in a week. In this article, we describe the Tox21 screening process, compound library preparation, data processing, and robotic system validation.
自 2008 年成立以来，美国 Tox21 机构间合作在开发和评估细胞模型以评估环境化学物质方面取得了重大进展，作为原理验证。目前，该计划已进入生产阶段（Tox21 第二阶段），最初侧重于核受体和应激反应途径的调节领域。在 Tox21 第二阶段，待测试的化学品数量已扩大到近 10000 种（10K）化合物，并实施了全自动化筛选平台。Tox21 机器人系统与信息学工作相结合，能够在一周内对 10K 种环境化学品进行三倍重复筛选和分析。在本文中，我们描述了 Tox21 的筛选过程、化合物库的制备、数据处理和机器人系统的验证。
