Wang Jin, Yang Guangbin, Chen Ying, Dai Yao, Yuan Qiaoling, Shan Qingyun, Pan Luzhao, Dai Li, Zou Xuexiao, Liu Feng, Xiong Cheng
College of Horticulture, Hunan Agricultural University, Changsha, China.
College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Front Genet. 2022 Feb 28;13:847328. doi: 10.3389/fgene.2022.847328. eCollection 2022.
The transcription factors, B-box (BBX), belong to a subfamily of the zinc finger family of proteins and exhibit multiple biological functions in plant growth, development, and abiotic stress response pathways. In this study, a total of 23 members were identified using the pepper reference genome database. According to the gene structure, conserved domains, and the phylogenetic tree, 23 genes were divided into four groups, wherein the analysis of the promoter region indicated the presence of -acting elements related to plant development, hormones, and stress response. Interspecies collinearity analysis showed that the had three duplicated gene pairs, and the highest gene density was found on chromosomes 2 and 7. Transcriptome RNA-seq data and quantitative polymerase chain reaction (qRT-PCR) analysis of pepper plants spanning the entire period showed that more than half of the genes were widely expressed in diversity tissues of pepper. Co-expression network analysis indicated that the and the anthocyanin structural genes had a close co-expression relationship. Thus, it was reasonably speculated that the genes may be involved in the regulation of anthocyanin biosynthesis. Overall, this study involved the genome-wide characterization of the family and may serve as a solid foundation for further investigations on genes involved in the anthocyanin synthesis mechanisms and development in pepper.
转录因子B-box（BBX）属于锌指蛋白家族的一个亚家族，在植物生长、发育和非生物胁迫响应途径中发挥多种生物学功能。在本研究中，利用辣椒参考基因组数据库共鉴定出23个成员。根据基因结构、保守结构域和系统发育树，将23个基因分为四组，其中启动子区域分析表明存在与植物发育、激素和胁迫响应相关的顺式作用元件。种间共线性分析表明，该家族有三对重复基因对，且在第2和第7号染色体上基因密度最高。对整个时期的辣椒植株进行转录组RNA测序数据和定量聚合酶链反应（qRT-PCR）分析表明，超过半数的该家族基因在辣椒的多种组织中广泛表达。共表达网络分析表明，该家族基因与花青素结构基因具有密切的共表达关系。因此，合理推测该家族基因可能参与花青素生物合成的调控。总体而言，本研究对该家族进行了全基因组特征分析，可为进一步研究辣椒中参与花青素合成机制和发育的该家族基因奠定坚实基础。
