McCarthy M, Lazzarini R A
J Gen Virol. 1982 Jan;58 Pt 1:205-9. doi: 10.1099/0022-1317-58-1-205.
Intracellular nucleocapsids, virtually free of host proteins and nucleic acids, were purified from cells acutely infected with several strains of mumps virus. VIral RNA obtained from the nucleocapsids was predominantly a single 50S species, with 28S RNA occurring as a secondary peak in the RNA profile. Nucleocapsids yielded approx. 9 micrograms 50S RNA per 10(9) infected cells. The 50S RNA was partially self-complementary, indicating that mumps viral nucleocapsids include full-length plus (+) sense antigenomes. Self-annealing and competition hybridization assays with 50S RNA suggest that 28S RNA includes duplexes consisting of full-length (+) and minus (-) sense 50S RNA. Properties of the viral RNA did not vary with the method of RNA isolation, time post-infection at which nucleocapsids were harvested, nor the strain of mumps virus.
从感染了几种腮腺炎病毒株的细胞中纯化出几乎不含宿主蛋白和核酸的细胞内核衣壳。从核衣壳获得的病毒RNA主要是单一的50S种类，28S RNA在RNA图谱中作为次要峰出现。每10⁹个感染细胞，核衣壳产生约9微克50S RNA。50S RNA部分自我互补，表明腮腺炎病毒核衣壳包含全长正（+）义反基因组。用50S RNA进行的自我退火和竞争杂交试验表明，28S RNA包括由全长（+）和负（-）义50S RNA组成的双链体。病毒RNA的特性不随RNA分离方法、收获核衣壳的感染后时间以及腮腺炎病毒株而变化。
