柳氮磺吡啶通过抑制破骨细胞融合分子 DC-STAMP 来抑制破骨细胞形成，而不影响成骨细胞的矿化。
Erucin inhibits osteoclast formation via suppressing cell-cell fusion molecule DC-STAMP without influencing mineralization by osteoblasts.
机构信息
Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan.
Department of Nutritional Sciences, Faculty of Nutritional Sciences, Tohto University, 4-2-7, Nishi, Kamishiba-cyo, Fukaya-shi, Saitama, 366-0052, Japan.
出版信息
BMC Res Notes. 2022 Mar 16;15(1):105. doi: 10.1186/s13104-022-05988-3.
OBJECTIVE
Erucin (ERN), an isothiocyanate, is derived from the vegetable arugula. Although ERN has antitumor and antioxidant activity, the effect of ERN on osteoclast and osteoblast differentiation is not well documented. In this study, we evaluated the effects of ERN on osteoclast and osteoblast differentiation in vitro.
RESULTS
ERN significantly reduced the formation of 1α,25(OH)D-induced tartrate-resistant acid phosphatase (TRAP)-positive cells at non-cytotoxic concentrations. Furthermore, ERN downregulated the mRNA expression of osteoclast-associated genes, such as nuclear factor of activated T cells cytoplasmic-1, TRAP, and cathepsin K. In addition, ERN suppressed mRNA expression of dendritic cell specific transmembrane protein (DC-STAMP), which encodes cell-cell fusion. However, ERN did not affect mineralization by osteoblasts. Thus, our data suggest that ERN may attenuate osteoclastic bone resorption by inhibiting multinucleation of mononuclear pre-osteoclasts and by suppressing mRNA expression of DC-STAMP in bone marrow cells without influencing mineralization by osteoblasts.
目的
萝卜硫素（ERN）是一种异硫氰酸盐，源自蔬菜芝麻菜。虽然 ERN 具有抗肿瘤和抗氧化活性，但 ERN 对破骨细胞和成骨细胞分化的影响尚未得到充分证实。在这项研究中，我们评估了 ERN 在体外对破骨细胞和成骨细胞分化的影响。
结果
ERN 在非细胞毒性浓度下显著减少了 1α,25(OH)D 诱导的抗酒石酸酸性磷酸酶（TRAP）阳性细胞的形成。此外，ERN 下调了破骨细胞相关基因的 mRNA 表达，如激活 T 细胞核因子细胞质-1、TRAP 和组织蛋白酶 K。此外，ERN 抑制了编码细胞-细胞融合的树突状细胞特异性跨膜蛋白（DC-STAMP）的 mRNA 表达。然而，ERN 并不影响成骨细胞的矿化。因此，我们的数据表明，ERN 可能通过抑制单核前破骨细胞的多核化和骨髓细胞中 DC-STAMP 的 mRNA 表达来抑制破骨细胞的骨吸收，而不影响成骨细胞的矿化。
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