活化诱导 SCAMP5 与 IFNα 在人浆细胞样树突状细胞中的共定位。

Activation-induced colocalisation of SCAMP5 with IFNα in human plasmacytoid dendritic cells.

机构信息

Rheumatology and Clinical Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands

Center for Translational Immunology, University Medical Centre Utrecht, Utrecht University, Utrecht, The Netherlands.

出版信息

Lupus Sci Med. 2022 Mar;9(1). doi: 10.1136/lupus-2022-000680.

DOI:10.1136/lupus-2022-000680

PMID:35296555

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8928376/

Abstract

INTRODUCTION

Plasmacytoid dendritic cells (pDCs) are the main producers of type I interferon (IFN) in SLE. pDCs express high secretory carrier membrane protein 5 (SCAMP5). Recent work in transfected HEK cells connects SCAMP5 to the type I IFN secretory pathway. To further study the role of SCAMP5 in IFNα secretion by pDCs, we focused on the subcellular distribution of SCAMP5 in human pDCs freshly isolated from peripheral blood.

METHODS

We measured SCAMP5 expression by flow cytometry in peripheral blood mononuclear cells of healthy subjects (n=8). Next, we assessed the colocalisation of SCAMP5 with IFNα in pDCs of healthy subjects (n=4) by evaluating bright detail similarity (BDS) scores using ImageStream technology.

RESULTS

We confirm that SCAMP5 is highly expressed by pDCs derived from peripheral blood. In activated pDCs, we show that SCAMP5 colocalises with IFNα (mean BDS 2.0±0.1; BDS >2.0 in 44% of pDCs).

CONCLUSION

SCAMP5 colocalises with IFNα in activated human pDCs, in support of a role of this trafficking protein in the secretion of type I IFN by pDCs.

摘要

简介

浆细胞样树突状细胞（pDCs）是 SLE 中 I 型干扰素（IFN）的主要产生细胞。pDCs 表达高分泌载体膜蛋白 5（SCAMP5）。最近在转染的 HEK 细胞中的研究将 SCAMP5 与 I 型 IFN 分泌途径联系起来。为了进一步研究 SCAMP5 在 pDCs IFNα 分泌中的作用，我们专注于从外周血中新鲜分离的人 pDCs 中 SCAMP5 的亚细胞分布。