Lebrun L, Tram C, d'Azambuja S, Pillot J
Acta Microbiol Hung. 1986;33(1):43-9.
This study was to determine by direct fluorescent antibody staining with antibodies prepared in rabbits, the degree of cross-reactions between serogroups of Legionella pneumophila (1 to 6) and the other antigenic species of Legionella (L. bozemanii, L. dumoffii, and L. micdadei), in order to increase the accuracy of diagnosis and to allow us to reduce the number of conjugates. The polyclonal antibodies were highly species and serogroup-specific without absorption for characterization of Legionella either in patient specimens or in isolated cultures by direct fluorescent antibody staining. No cross-reaction was observed with non-legionella bacteria isolated from sputum specimens. A battery of conjugates for different serogroups and species is necessary for increasing the accuracy of diagnosis of legionellosis.
本研究旨在通过用兔制备的抗体进行直接荧光抗体染色，确定嗜肺军团菌血清群（1至6）与军团菌其他抗原种（博兹曼军团菌、杜莫夫军团菌和米克戴德军团菌）之间的交叉反应程度，以提高诊断准确性并减少所用结合物的数量。这些多克隆抗体具有高度的种属和血清群特异性，无需吸收即可通过直接荧光抗体染色对患者标本或分离培养物中的军团菌进行鉴定。未观察到与从痰标本中分离出的非军团菌细菌发生交叉反应。为提高军团菌病诊断的准确性，需要一系列针对不同血清群和种的结合物。
