鉴定西方马脑炎病毒在其自然传播媒介——库蚊（Culex tarsalis）中的唾液腺逃逸屏障。
Identification of salivary gland escape barriers to western equine encephalitis virus in the natural vector, Culex tarsalis.
机构信息
Laboratory of Vector-Borne Diseases, Division of Viral Products, Office of Vaccine Research and Review, Food and Drug Administration, White Oak, Maryland, United States of America.
Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, United States of America.
出版信息
PLoS One. 2022 Mar 17;17(3):e0262967. doi: 10.1371/journal.pone.0262967. eCollection 2022.
Herein we describe a previously uninvestigated salivary gland escape barrier (SEB) in Culex tarsalis mosquitoes infected with two different strains of Western equine encephalitis virus (WEEV). The WEEV strains were originally isolated either from mosquitoes (IMP181) or a human patient (McMillan). Both IMP181 and McMillan viruses were fully able to infect the salivary glands of Culex tarsalis after intrathoracic injection as determined by expression of mCherry fluorescent protein. IMP181, however, was better adapted to transmission as measured by virus titer in saliva as well as transmission rates in infected mosquitoes. We used chimeric recombinant WEEV strains to show that inclusion of IMP181-derived structural genes partially circumvents the SEB.
在这里，我们描述了一种以前未被研究过的库蚊唾液腺逃逸屏障（SEB），该屏障感染了两种不同株系的西部马脑炎病毒（WEEV）。这两种 WEEV 株系最初分别从蚊子（IMP181）或人类患者（McMillan）中分离出来。通过表达 mCherry 荧光蛋白，经胸内注射后，两种 IMP181 和 McMillan 病毒都完全能够感染库蚊的唾液腺。然而，IMP181 更适合传播，这可以通过唾液中的病毒滴度以及感染蚊子中的传播率来衡量。我们使用嵌合重组 WEEV 株系表明，包含 IMP181 衍生的结构基因部分绕过了 SEB。
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