Department of Agricultural, Food, Environmental and Animal Sciences, Via delle Scienze 206, University of Udine, 33100, Udine, Italy.
Department of Agricultural, Food, Environmental and Animal Sciences, Via delle Scienze 206, University of Udine, 33100, Udine, Italy; Department of Life Sciences, University of Trieste, Via Licio Giorgieri, 5, 34127, Trieste, Italy.
J Plant Physiol. 2022 Apr;271:153659. doi: 10.1016/j.jplph.2022.153659. Epub 2022 Mar 1.
Phytoplasmas are sieve-elements restricted wall-less, pleomorphic pathogenic microorganisms causing devastating damage to over 700 plant species worldwide. The invasion of sieve elements by phytoplasmas has several consequences on nutrient transport and metabolism, anyway studies about changes of the mineral-nutrient profile following phytoplasma infections are scarce and offer contrasting results. Here, we examined changes in macro- and micronutrient concentration in tomato plant upon 'Candidatus Phytoplasma solani' infection. To investigate possible effects of 'Ca. P. solani' infection on mineral element allocation, the mineral elements were separately analysed in leaf midrib, leaf lamina and root. Moreover, we focused our analysis on the transcriptional regulation of genes encoding trans-membrane transporters of mineral nutrients. To this aim, a manually curated inventory of differentially expressed genes encoding transporters in tomato leaf midribs was mined from the transcriptional profile of healthy and infected tomato leaf midribs. Results highlighted changes in ion homeostasis in the host plant, and significant modulations at transcriptional level of genes encoding ion transporters and channels.
植原体是一种无壁、多形的筛管限制性病原体，对全球超过 700 种植物物种造成严重破坏。植原体对筛管的入侵对养分运输和代谢有几个影响，然而，关于植原体感染后矿物质-养分谱变化的研究很少，且结果相互矛盾。在这里，我们研究了感染“候选植原体 solani”后番茄植株中大量和微量元素浓度的变化。为了研究“Ca. P. solani”感染可能对矿物质元素分配的影响，将矿物质元素分别分析在叶中脉、叶片和根中。此外，我们还专注于分析编码矿物质养分跨膜转运蛋白的基因的转录调控。为此，从健康和感染的番茄叶中脉的转录谱中挖掘了一个手动整理的差异表达基因编码转运体的清单。结果突出了宿主植物中离子动态平衡的变化，以及编码离子转运体和通道的基因在转录水平上的显著调节。
