Sarkar Rajesh Dev, Kalita Mohan Chandra
Department of Biotechnology, Gauhati University, Guwahati, Assam, India.
Heliyon. 2022 Mar 8;8(3):e09076. doi: 10.1016/j.heliyon.2022.e09076. eCollection 2022 Mar.
Selenium Nanoparticles (SeNPs) exhibit tremendous application in agriculture as antimicrobials or as nano fertilizer. Present work reports the eco-friendly synthesis of SeNPs by using L. flower extract (aqueous) as a reducing/capping agent and selenium dioxide as a precursor. The method used here is free of any toxic reducing agents and organic solvents. The synthesis process of SeNPs took 5 h at 60 °C, confirmed by the brick red colour of the solution followed by UV-Vis spectroscopy and further characterized by XRD, FTIR, EDX and SEM. The average size (diameter) of the SeNPs were found to be 60.31 nm by DLS. It has shown strong antimicrobial activity against and at 2.5, 5 and 10 mg/mL concentrations. Besides, its application improved seed germination and growth parameters of (TS 36 variety) under salt stress. 25 mg/L SeNPs has improved the germination percentage by around 31%, shoot length by 92%, root length by 78% and total chlorophyll content by 49% under 200 mM NaCl stress. This SeNPs could be a potential antimicrobial agent in treating plant diseases caused by the mentioned phytopathogens, having no or minimum toxicity, in fact having positive impacts on plant growth.
硒纳米颗粒（SeNPs）在农业中作为抗菌剂或纳米肥料展现出巨大的应用潜力。目前的工作报道了以L.花提取物（水溶液）作为还原剂/封端剂、二氧化硒作为前驱体，通过生态友好的方法合成SeNPs。这里使用的方法不含任何有毒还原剂和有机溶剂。SeNPs的合成过程在60℃下耗时5小时，溶液呈现砖红色，通过紫外可见光谱法得以证实，随后通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、能量色散X射线光谱（EDX）和扫描电子显微镜（SEM）进一步表征。通过动态光散射（DLS）测定，SeNPs的平均尺寸（直径）为60.31纳米。在2.5、5和10毫克/毫升的浓度下，它对[具体菌种1]和[具体菌种2]表现出强大的抗菌活性。此外，在盐胁迫下，其应用改善了[具体植物品种（TS 36品种）]的种子萌发和生长参数。在200毫摩尔/升氯化钠胁迫下，25毫克/升的SeNPs使发芽率提高了约31%，茎长提高了92%，根长提高了78%，总叶绿素含量提高了49%。这种SeNPs可能是一种潜在的抗菌剂，可用于治疗由上述植物病原体引起的植物疾病，无毒或毒性极小，实际上对植物生长有积极影响。
