Díaz-Rúa Adrián, Chivite Mauro, Comesaña Sara, Conde-Sieira Marta, Soengas José L
Centro de Investigación Mariña, Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, Vigo, Spain.
Front Physiol. 2022 Mar 1;13:800218. doi: 10.3389/fphys.2022.800218. eCollection 2022.
We hypothesize that opioids are involved in the regulation of food intake in fish through homeostatic and hedonic mechanisms. Therefore, we evaluated in rainbow trout () hypothalamus and telencephalon changes in precursors, endogenous ligands and receptors of the opioid system under different situations aimed to induce changes in the homeostatic (through fasted/fed/refed fish) and hedonic (through feeding fish a control or a palatable high-fat diet) regulation of food intake. No major changes occurred in parameters assessed related with the nutritional condition of fish (fasted/fed/refed), allowing us to suggest that the opioid system seems not to have an important role in the homeostatic regulation of food intake in rainbow trout. The responses observed in telencephalon of rainbow trout fed the palatable high-fat diet included a decrease in mRNA abundance of the opioid precursor , in a way similar to that known in mammals, and increased mRNA abundance of the opioid receptors and supporting a role for telencephalic opioid system in the hedonic regulation of food intake in fish.
我们假设阿片类物质通过稳态和享乐机制参与鱼类食物摄入的调节。因此，我们评估了虹鳟鱼下丘脑和端脑中阿片系统的前体、内源性配体和受体在不同情况下的变化，这些情况旨在诱导食物摄入的稳态调节（通过禁食/喂食/再喂食的鱼）和享乐调节（通过给鱼喂食对照或美味的高脂肪饮食）发生变化。与鱼类营养状况（禁食/喂食/再喂食）相关的评估参数没有发生重大变化，这使我们认为阿片系统似乎在虹鳟鱼食物摄入的稳态调节中没有重要作用。给虹鳟鱼喂食美味高脂肪饮食后，在其端脑中观察到的反应包括阿片前体的mRNA丰度降低，这与哺乳动物中已知的情况类似，并且阿片受体和的mRNA丰度增加，这支持了端脑阿片系统在鱼类食物摄入的享乐调节中的作用。
