Pietsch Constanze, Pawlak Paulina, Konrad Jonathan
School of Agricultural, Forest and Food Sciences (HAFL), Bern University of Applied Sciences (BFH), 3052 Zollikofen, Switzerland.
Animals (Basel). 2024 Nov 26;14(23):3413. doi: 10.3390/ani14233413.
Changes in gene expression in carps' brains over time following acute stressors has not been studied in detail so far. Consequently, a stress trial with juvenile common carp was conducted to investigate transcriptomic differences in four brain parts in response to acute negative stressors and feed reward, focusing on appetite-related genes, serotonergic and dopaminergic pathways, and other involved systems, at 30, 60, and 90 min after treatments. The treatments showed pronounced effects on the gene expression patterns across brain parts compared to control fish. Notably, expression increased in the telencephalon following negative stressors and feed reward, suggesting a stress-coping mechanism by promoting food intake. Unlike zebrafish, expression in carp showed varying responses, indicating species-specific regulation of appetite and stress. Serotonergic and dopaminergic pathways were also affected, with alterations in the respective receptors' expression, confirming their roles in stress and reward processing. Additionally, this study highlights the involvement of the opioid- and gamma-aminobutyric acid systems in stress and feeding regulation across brain parts. Furthermore, principal component analyses revealed that neurotransmitter levels in the different brain parts contribute to the explained variance. These findings deepen our understanding of how different fish species react to acute stress and rewards.
迄今为止，尚未详细研究鲤鱼大脑在急性应激源作用下随时间的基因表达变化。因此，对幼龄普通鲤鱼进行了一项应激试验，以研究在处理后30、60和90分钟时，四个脑区对急性负面应激源和饲料奖励的转录组差异，重点关注与食欲相关的基因、血清素能和多巴胺能途径以及其他相关系统。与对照鱼相比，处理对各脑区的基因表达模式有显著影响。值得注意的是，在负面应激源和饲料奖励后，端脑的表达增加，表明通过促进食物摄入的应激应对机制。与斑马鱼不同，鲤鱼的表达表现出不同的反应，表明食欲和应激存在物种特异性调节。血清素能和多巴胺能途径也受到影响，各自受体的表达发生改变，证实了它们在应激和奖励处理中的作用。此外，本研究强调了阿片样物质和γ-氨基丁酸系统在各脑区应激和摄食调节中的参与。此外，主成分分析表明，不同脑区的神经递质水平有助于解释方差。这些发现加深了我们对不同鱼类如何应对急性应激和奖励的理解。
