Ukil Bidisha, Joardar Nikhilesh, Babu Santi Prasad Sinha, Lyndem Larisha M
Parasitology Research Laboratory, Department of Zoology, Visva Bharati, Santiniketan, West Bengal 731235 India.
Molecular Parasitology Laboratory, Department of Zoology, Visva Bharati, Santiniketan, West Bengal 731235 India.
J Parasit Dis. 2022 Mar;46(1):139-151. doi: 10.1007/s12639-021-01415-9. Epub 2021 Aug 2.
The peculiarity of energy metabolism in helminths is the ability to undergo transition from aerobic to anaerobic under low oxygen tension. during its adult stage. Fumarate reductase and succinate dehydrogenase of mitochondria are the two enzymes responsible during this transition and adaptation to this hypoxic environment. Earlier we had reported that three species of plant, , and altered the morphology, ionic concentration and neurotransmission of the cestode parasite The present study aimed at exploring the mechanism of leaf extracts of the three plant species of on the mitochondrial activity of the parasite that chiefly involve the NADH-fumarate reductase system which is the terminal step in phosphoenolpyruvate carboxykinase succinate pathway. The structure of mitochondria was observed through electron microsopy and its density was detected through confocal microscopy, spectroflourimetry and spectrophotometry, while enzyme activities were assayed through native gel and spectrophotometric assays. Praziquantel was tested on the parasites as a reference drug to compare its effects with that of the plant extracts. The mitochondria architecture was altered, and enzymes activity decraeased by 60% in all three plant species of treated parasites which suggested that these three species posses potent chemotherapeutic properties.
The online version contains supplementary material available at 10.1007/s12639-021-01415-9.
蠕虫能量代谢的特点是在低氧张力下能够从需氧转变为厌氧，在其成虫阶段。线粒体中的延胡索酸还原酶和琥珀酸脱氢酶是这种转变和适应这种低氧环境过程中的两种关键酶。此前我们报道过三种植物，[植物名称1]、[植物名称2]和[植物名称3]改变了绦虫寄生虫的形态、离子浓度和神经传递。本研究旨在探索这三种植物的叶提取物对寄生虫线粒体活性的作用机制，这主要涉及磷酸烯醇式丙酮酸羧激酶 - 琥珀酸途径中的末端步骤NADH - 延胡索酸还原酶系统。通过电子显微镜观察线粒体的结构，并通过共聚焦显微镜、荧光光谱法和分光光度法检测其密度，同时通过天然凝胶和分光光度法测定酶活性。以吡喹酮作为参考药物对寄生虫进行测试，以比较其与植物提取物的效果。在所有三种经[植物名称]处理的寄生虫中，线粒体结构发生改变，酶活性降低了60%，这表明这三种[植物名称]具有强大的化疗特性。
在线版本包含可在10.1007/s12639 - 021 - 01415 - 9获取的补充材料。
