Effect of plant on the mitochondrial activity of .

UNLABELLED

The peculiarity of energy metabolism in helminths is the ability to undergo transition from aerobic to anaerobic under low oxygen tension. during its adult stage. Fumarate reductase and succinate dehydrogenase of mitochondria are the two enzymes responsible during this transition and adaptation to this hypoxic environment. Earlier we had reported that three species of plant, , and altered the morphology, ionic concentration and neurotransmission of the cestode parasite The present study aimed at exploring the mechanism of leaf extracts of the three plant species of on the mitochondrial activity of the parasite that chiefly involve the NADH-fumarate reductase system which is the terminal step in phosphoenolpyruvate carboxykinase succinate pathway. The structure of mitochondria was observed through electron microsopy and its density was detected through confocal microscopy, spectroflourimetry and spectrophotometry, while enzyme activities were assayed through native gel and spectrophotometric assays. Praziquantel was tested on the parasites as a reference drug to compare its effects with that of the plant extracts. The mitochondria architecture was altered, and enzymes activity decraeased by 60% in all three plant species of treated parasites which suggested that these three species posses potent chemotherapeutic properties.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12639-021-01415-9.