Department of Nutrition and Food Hygiene, Tianjin Institute of Environmental and Operational Medicine, Tianjin, P. R. China.
Department of Nutrition and Food Hygiene, Tianjin Institute of Environmental and Operational Medicine, Tianjin, P. R. China; Department of Nutrition and Food Hygiene, Binzhou Medical University, Yantai, P. R. China.
Nutr Res. 2022 May;101:1-13. doi: 10.1016/j.nutres.2022.01.005. Epub 2022 Feb 18.
The gut microbiota can affect the intestinal barrier and other physiological functions. Tangerine pith contains various citrus pectins, which modulate the gut microbiota in a structure-dependent manner. We hypothesized that tangerine pith extract (TPE) has prebiotic effects on gut dysbiosis in antibiotic-treated mice. TPE was prepared via acid hydrolysis and isolated using an ultrafiltration method. Gut dysbiosis was induced through treatment with antibiotic cocktail for 7 days in mice. After spontaneous recovery or being supplemented with 0.5 mL TPE for 14 days, the gut microbiota composition and other parameters were examined. The 16s ribosomal DNA high-throughput sequencing and analysis confirmed that TPE positively improved the gut microbiota. The relative abundance of some probiotics such as [Ruminococcus]_torques_group, [Clostridium]_innocuum_group, and Enterorhabdus were significantly increased and harmful bacteria such as Alistipes were decreased in TPE-supplemented mice. Furthermore, the production of propionate and butyrate in the colonic contents were significantly enhanced in the TPE-treated mice. Serum D-lactic acid and diamine oxidase activity were increased in the antibiotic treatment group and reversed in the TPE-treated group. Moreover, protein expression of intestinal occludin was significantly increased by TPE. In conclusion, TPE is effective in restoring the gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.
肠道微生物群可以影响肠道屏障和其他生理功能。桔皮含有各种柑橘果胶，以结构依赖的方式调节肠道微生物群。我们假设桔皮提取物（TPE）对接受抗生素治疗的小鼠肠道菌群失调具有益生元作用。TPE 通过酸水解制备，并通过超滤方法分离。通过用抗生素鸡尾酒处理 7 天在小鼠中诱导肠道菌群失调。在自发恢复或补充 0.5 mL TPE 14 天后，检查肠道微生物群组成和其他参数。16s 核糖体 DNA 高通量测序和分析证实 TPE 可积极改善肠道微生物群。一些益生菌如[Ruminococcus]_torques_group、[Clostridium]_innocuum_group 和 Enterorhabdus 的相对丰度显著增加，而 TPE 补充组中的有害细菌如 Alistipes 减少。此外，TPE 处理组的结肠内容物中丙酸盐和丁酸盐的产量显著增加。抗生素治疗组血清 D-乳酸和二胺氧化酶活性增加，TPE 治疗组恢复正常。此外，TPE 显著增加了肠道闭合蛋白的蛋白表达。总之，TPE 可有效恢复抗生素治疗后小鼠的肠道微生物群，并可作为防治肠道菌群失调的益生元。
