Mei Hong, Yang Shaoping, Peng An'na, Li Ruizhen, Xiang Feiyan, Zheng Hao, Tan Yafei, Zhang Ya, Zhou Ai'fen, Zhang Jianduan, Xiao Han
Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Maternal and Child Health Care, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Front Microbiol. 2022 Aug 18;13:891679. doi: 10.3389/fmicb.2022.891679. eCollection 2022.
The gut microbiota undergoes rapid and vital changes to microbial community structure and the microbial-immune crosstalk during the first 3 years of life, which is thought to be involved in the pathobiology of later-life disease. Compared to single-born children, little is known about the gut microbiota of twins in early childhood. Based on the Wuhan Twin Birth Cohort study, 344 stool samples from 204 twin families were analyzed to investigate the difference in gut microbiota composition at 6, 12, and 24 months of age. Furthermore, this study evaluated the association between gut microbiota development curves and body mass index z-score (BMI_Z) curves at 6, 12, and 24 months of age. The predominant microbiota phyla identified in twins were , and . The richness and diversity of gut microbiota increased from 6 to 24 months old (alpha diversity with < 0.05). Beta diversity revealed 61 gut microbiota genera that were significantly different in relative abundance among the three age groups. Among the 61 gut microbiota genera, 30 distinct trajectory curves (DTCs) were generated by group-based trajectory models after log2 transformation of their relative abundance. Subsequently, Spearman correlation analysis revealed that only five gut microbiota DTC were correlated with the BMI_Z DTC. Therefore, we further examined the association between the five gut microbiota genera DTC and BMI_Z DTC using generalized estimation equation models. The results revealed a significant association between the DTC groups of and that of BMI_Z (coefficient = 0.75, = 0.04). The results of this study validated the hypothesis that the richness and diversity of gut microbiota developed with age in twins. Moreover, participants with a higher DTC of log2-transformed had a higher BMI_Z DTC during the first 2 years of life. Further studies are needed to confirm the association between and BMI_Z in other populations.
在生命的最初3年中，肠道微生物群经历了微生物群落结构和微生物-免疫相互作用的快速且至关重要的变化，这被认为与晚年疾病的病理生物学有关。与单胎出生的儿童相比，幼儿期双胞胎的肠道微生物群情况鲜为人知。基于武汉双胞胎出生队列研究，对来自204个双胞胎家庭的344份粪便样本进行了分析，以调查6、12和24月龄时肠道微生物群组成的差异。此外，本研究评估了6、12和24月龄时肠道微生物群发育曲线与体重指数z评分（BMI_Z）曲线之间的关联。在双胞胎中鉴定出的主要微生物门类为 、 和 。肠道微生物群的丰富度和多样性从6月龄到24月龄增加（α多样性， ＜0.05）。β多样性显示，61个肠道微生物属在三个年龄组中的相对丰度存在显著差异。在这61个肠道微生物属中，基于组的轨迹模型在对其相对丰度进行log2转换后生成了30条不同的轨迹曲线（DTC）。随后，Spearman相关性分析显示，只有5条肠道微生物DTC与BMI_Z DTC相关。因此，我们使用广义估计方程模型进一步研究了这5个肠道微生物属DTC与BMI_Z DTC之间的关联。结果显示， 和BMI_Z的DTC组之间存在显著关联（系数 = 0.75， = 0.04）。本研究结果验证了双胞胎肠道微生物群丰富度和多样性随年龄增长而发展的假设。此外，在生命的前两年中，log2转换后的 DTC较高的参与者其BMI_Z DTC也较高。需要进一步研究以证实其他人群中 与BMI_Z之间的关联。
