Bahauddin Zakariya University, Multan, Pakistan.
Mol Biol Rep. 2022 May;49(5):3903-3910. doi: 10.1007/s11033-022-07240-4. Epub 2022 Mar 17.
Population structure and genetic diversity of bronze featherback Notopterus notopterus, fish was not studied yet from Pakistan. So, genetic diversity and population structure of N. notopterus was analysed using two mitochondrial DNA genetic markers, ATPase 6/8 and Cytochrome b.
150 specimens were collected from five different rivers of Pakistan, resulting 56 haplotypes were detected for ATPase 6/8, Cytb and concatenated gene. Haplotype and nucleotide diversity for ATPase 6/8, Cytb and concatenated gene was observed below 1% among five natural populations of N. notopterus. ATPase 6/8 and Cytb genetic variance among populations was 6% and among and within individuals was 94%. Concatenated genetic variance among populations was 11%, among individual 5% and within individuals 84%. Fst value among all population was found 0.091 (p-value=0.02, p<0.05). The combined data set mean coefficient of genetic differentiation (F) was 0.5572. The pair-wise F was 0.000 (Chenab) to 0.88911 (Ravi). Maximum likelihood phylogeographic history of concatenated gene haplotypes showed four distinct diversified clusters. AMOVA, PCoA (Principal Coordinate Analysis) and maximum likelihood tree indicated that the natural populations of N. notopterus were comprised of four genetic stocks among five Pakistani rivers (Chenab, Indus, Jehlum, Ravi and Satluj).
This study provides the higher level of genetic diversity with confirmatory proofs among genetic stocks of five natural populations of N. notopterus. The information of genetic diversity and genetic variation, from this research can be utilised to help conserve and manage the species in the wild.
青铜鱼（Notopterus notopterus）的种群结构和遗传多样性尚未在巴基斯坦进行研究。因此，本研究使用两种线粒体 DNA 遗传标记 ATPase 6/8 和 Cytb 分析了 N. notopterus 的遗传多样性和种群结构。
从巴基斯坦的五条不同河流采集了 150 个样本，共检测到 ATPase 6/8、Cytb 和串联基因的 56 个单倍型。五个自然种群的 N. notopterus 的 ATPase 6/8、Cytb 和串联基因的单倍型和核苷酸多样性均低于 1%。ATPase 6/8 和 Cytb 基因的遗传方差在种群间为 6%，在个体间为 94%。串联基因的遗传方差在种群间为 11%，个体间为 5%，个体内为 84%。所有种群的 Fst 值为 0.091（p 值=0.02，p<0.05）。综合数据集的平均遗传分化系数（F）为 0.5572。成对 F 值在 0.000（Chenab）到 0.88911（Ravi）之间。串联基因单倍型的最大似然系统发育历史显示出四个明显分化的聚类。AMOVA、PCoA（主坐标分析）和最大似然树表明，在巴基斯坦的五条河流（Chenab、Indus、Jehlum、Ravi 和 Satluj）中，N. notopterus 的自然种群由四个遗传群体组成。
本研究在 N. notopterus 的五个自然种群的遗传群体中提供了更高水平的遗传多样性和确认证据。这项研究的遗传多样性和遗传变异信息可用于帮助保护和管理野外的物种。
