National Bureau of Fish Genetic Resources, Lucknow, India.
Amity Institute of Biotechnology, Amity University, Lucknow Campus, Lucknow, India.
Mol Biol Rep. 2020 Nov;47(11):8579-8592. doi: 10.1007/s11033-020-05901-w. Epub 2020 Oct 20.
Genetic diversity and population structure in Indian featherback fish, Chitala chitala (Hamilton, 1822) was investigated by combined analyses of two full mitochondrial genes, ATPase 6/8 and Cytochrome b. A total of 403 individuals, collected from 14 rivers yielded 61 haplotypes. Hierarchical partitioning analysis identified 19.01% variance 'among' and 80.99% variance 'within groups and populations'. The mean coefficient of genetic differentiation (F) was observed to be significant 0.26 (p < 0.05). Mantel tests rejected the hypothesis that genetic and geographic distances were correlated. The patterns of genetic differentiation, AMOVA and principal coordinate analyses indicated that natural populations were sub-structured and comprised of four genetic stocks of C. chitala in Indian rivers. The results also supported the higher resolution potential of concatenated gene sequences. The knowledge of genetic variation and divergence, from this study, can be utilized for its scientific conservation and management in the wild.
采用联合分析两种完整的线粒体基因（ATPase 6/8 和 Cytochrome b），研究了印度羽鳃鲐（Chitala chitala (Hamilton, 1822)）的遗传多样性和种群结构。从 14 条河流中采集了 403 条个体，共获得 61 种单倍型。分层分区分析确定了 19.01%的方差“在群体和种群之间”，80.99%的方差“在群体内”。遗传分化系数（F）的平均值观察到显著（p<0.05）。Mantel 检验拒绝了遗传距离和地理距离相关的假设。遗传分化模式、AMOVA 和主坐标分析表明，自然种群是亚结构的，由印度河流中的四个 C. chitala 遗传种群组成。研究结果还支持了串联基因序列的更高分辨率潜力。本研究的遗传变异和分化知识可用于其在野外的科学保护和管理。
