Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
School of Traditional Chinese Medicine, Capital Medical University, Beijing, China.
Biomed Chromatogr. 2022 Jul;36(7):e5374. doi: 10.1002/bmc.5374. Epub 2022 Mar 30.
Diabetic peripheral neuropathy (DPN) is one of the most common complications of diabetes with limited therapies. Tang Luo Ning (TLN), a traditional Chinese medicine compound, has been proved to be effective in the treatment of DPN in clinical and experimental studies. However, the potential metabolic mechanism of TLN for the treatment of DPN is still unclear. Here the therapeutic effect of TLN on DPN was studied, and HPLC-IT-TOF/MS was used to explore the metabolic changes related to DPN and to explore the mechanism of TLN on DPN induced by high glucose. Furthermore, metabolic pathway analysis was used to explore the metabolic changes induced by DPN and TLN. As a result, TLN could improve the peripheral nerve function of DPN rats, and TLN could reduce the demyelination of the sciatic nerve in DPN rats. Metabolomics analysis showed that 14 potential biomarkers (citrate, creatine, fumarate, glyceric acid, glycine, succinate, etc.) of both DPN and TLN treatment were identified. Pathway analysis showed that the changes in these metabolites were mainly related to the citrate cycle (TCA cycle); glycine, serine, and threonine metabolism; and glyoxylate and dicarboxylate metabolism.
糖尿病周围神经病变（DPN）是糖尿病最常见的并发症之一，目前治疗方法有限。中药复方糖络宁（TLN）在临床和实验研究中已被证明对 DPN 治疗有效。然而，TLN 治疗 DPN 的潜在代谢机制尚不清楚。本研究探讨了 TLN 对 DPN 的治疗作用，采用 HPLC-IT-TOF/MS 技术探讨与 DPN 相关的代谢变化，并探讨 TLN 对高糖诱导的 DPN 的作用机制。此外，还采用代谢通路分析方法探讨 DPN 和 TLN 诱导的代谢变化。结果表明，TLN 可改善 DPN 大鼠的周围神经功能，减轻 DPN 大鼠坐骨神经脱髓鞘。代谢组学分析共鉴定出 14 种 DPN 和 TLN 治疗的潜在生物标志物（柠檬酸、肌酸、富马酸、甘油酸、甘氨酸、琥珀酸等）。通路分析表明，这些代谢物的变化主要与柠檬酸循环（TCA 循环）、甘氨酸、丝氨酸和苏氨酸代谢以及乙醛酸和二羧酸代谢有关。
