θ–γ 神经振荡刺激海马 CA3 区对大鼠空间认知能力的影响。
Effect of θ–γ neural oscillation stimulation in hippocampal CA3 area on spatial cognition ability in rats.
机构信息
Department of Physiology, Wannan Medical College, Wuhu 241002, Anhui Province, China
Clinical Medical College, Wannan Medical College, Wuhu 241002, Anhui Province, China
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2021 Dec 25;50(6):762-769. doi: 10.3724/zdxbyxb-2021-0165.
Objective: To investigate the effects of θ–γ neural oscillation stimulation in hippocampal CA3 area on spatial cognition ability in rats. Methods: According to the results of Y maze shock avoidance training, the rats were divided into fast avoidance response group and general avoidance response group. Using endogenous θ–γ neural oscillations from the fast avoidance response rats to perform deep brain stimulation in vivo to the left and right hippocampal CA3 region of rats with general avoidance response, then the spatial cognition was tested by Y maze shock avoidance training. The variation of θ oscillation and low-γ neural oscillation phase-amplitude coupling (PAC) in CA3 area was analyzed by wavelet packet extraction technique. Western blotting was used to detect the expression of N-methyl-D-aspartate receptor 2B subunit (NR2B) and postsynaptic density(PSD)-95 in hippocampal tissues of rats to explore its molecular mechanism. Results: Compared with the general avoidance response rats, the days to reach the standard, the training number, the correct response time and the error reaction number in simulated stimulus avoidance response rats were significantly reduced, but the correct response rate was significantly increased (all P<0.01); the θ–γ neural oscillations PAC in the hippocampal CA3 region in the simulated stimulus avoidance response rats (3–5 Hz and 30–34, 38–42, 44–48 Hz; 5–7 Hz and 42–46, 44–48, 54–58 Hz) were significantly higher than that in the general avoidance response rats (all P<0.05). Meanwhile, the protein expressions of NR2B and PSD-95 in hippocampal tissues were significantly increased (both P<0.05) in simulated stimulus avoidance response rats. Conclusion: The spatial cognition of normal avoidance response rats can be significantly improved by endogenous θ–γ neural oscillation stimulation to hippocampal CA3 region, which may be caused by the enhancement of synaptic plasticity mediated by NR2B and PSD-95.
目的
探讨海马 CA3 区θ-γ 神经振荡刺激对大鼠空间认知能力的影响。方法：根据 Y 迷宫避错训练结果，将大鼠分为快速避错反应组和一般避错反应组。利用快速避错反应大鼠的内源性θ-γ 神经振荡，对一般避错反应大鼠的左右海马 CA3 区进行体内深部脑刺激，然后通过 Y 迷宫避错训练测试大鼠的空间认知能力。采用小波包提取技术分析 CA3 区θ 振荡和低γ 神经振荡相位-幅度耦合（PAC）的变化。采用 Western blotting 检测大鼠海马组织中 N-甲基-D-天冬氨酸受体 2B 亚基（NR2B）和突触后密度（PSD）-95 的表达，探讨其分子机制。结果：与一般避错反应大鼠相比，模拟刺激避错反应大鼠达到标准所需天数、训练次数、正确反应时间和错误反应次数均明显减少，但正确反应率明显增加（均 P<0.01）；模拟刺激避错反应大鼠海马 CA3 区θ-γ 神经振荡 PAC（3-5 Hz 和 30-34、38-42、44-48 Hz；5-7 Hz 和 42-46、44-48、54-58 Hz）明显高于一般避错反应大鼠（均 P<0.05）。同时，模拟刺激避错反应大鼠海马组织中 NR2B 和 PSD-95 的蛋白表达明显增加（均 P<0.05）。结论：内源性θ-γ 神经振荡刺激海马 CA3 区可显著改善正常避错反应大鼠的空间认知能力，这可能是由 NR2B 和 PSD-95 介导的突触可塑性增强所致。
Zotero 插件